CLONING AND EXPRESSION OF CDNAS FROM ENTERICALLY-TRANSMITTED NON-A, NON-B HEPATITIS-VIRUS

被引：15

作者：

ICHIKAWA, M ^{[1
]}

ARAKI, M ^{[1
]}

RIKIHISA, T ^{[1
]}

UCHIDA, T ^{[1
]}

SHIKATA, T ^{[1
]}

MIZUNO, K ^{[1
]}

机构：

[1] NIHON UNIV,SCH MED,DEPT PATHOL,ITABASHI KU,TOKYO 173,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1991年 / 35卷 / 07期

关键词：

D O I：

10.1111/j.1348-0421.1991.tb01584.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The fragment gene of enterically-transmitted non-A, non-B hepatitis virus (ET-NANBHV) was cloned as a cDNA and inserted into an expression vector pUEX2. The recombinant protein was expressed in Escherichia coli HB101 as a fusion protein with beta-galactosidase (beta-Gal). The fusion protein reacted with the sera of infected cynomolgus monkeys and of patients from Myanmar. This reaction was highly related with ET-NANBHV infection, and obviously demonstrates in that the recombinant protein can be used for the detection of ET-NANBHV infection.

引用

页码：535 / 543

页数：9

共 9 条

[1] REPORT OF A WORKSHOP - WATERBORNE NON-A, NON-B HEPATITIS [J].

GUST, ID ;

PURCELL, RH .

JOURNAL OF INFECTIOUS DISEASES, 1987, 156 (04) :630-635

[2]

KANE MA, 1984, JAMA-J AM MED ASSOC, V252, P3140, DOI 10.1001/jama.252.22.3140

[3] A CLINICAL AND EPIDEMIOLOGICAL-STUDY OF AN EPIDEMIC OF NON-A NON-B HEPATITIS IN RANGOON [J].

MYINT, H ;

SOE, MM ;

KHIN, T ;

MYINT, TM ;

TIN, KM .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1985, 34 (06) :1183-1189

[4] ISOLATION OF A CDNA FROM THE VIRUS RESPONSIBLE FOR ENTERICALLY TRANSMITTED NON-A, NON-B HEPATITIS [J].

REYES, GR ;

PURDY, MA ;

KIM, JP ;

LUK, KC ;

YOUNG, LM ;

FRY, KE ;

BRADLEY, DW .

SCIENCE, 1990, 247 (4948) :1335-1339

[5] ENZYMATIC AMPLIFICATION OF BETA-GLOBIN GENOMIC SEQUENCES AND RESTRICTION SITE ANALYSIS FOR DIAGNOSIS OF SICKLE-CELL ANEMIA [J].

SAIKI, RK ;

SCHARF, S ;

FALOONA, F ;

MULLIS, KB ;

HORN, GT ;

ERLICH, HA ;

ARNHEIM, N .

SCIENCE, 1985, 230 (4732) :1350-1354

[6] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[7]

SOE S, 1989, LIVER, V9, P135

[8]

UCHIDA T, 1990, JPN J EXP MED, V60, P23

[9]

WONG DC, 1980, LANCET, V2, P876

← 1 →