OVEREXPRESSION OF THE HYALURONAN RECEPTOR RHAMM IS TRANSFORMING AND IS ALSO REQUIRED FOR H-RAS TRANSFORMATION

被引：266

作者：

HALL, CL ^{[1
]}

YANG, BH ^{[1
]}

YANG, XW ^{[1
]}

ZHANG, SW ^{[1
]}

TURLEY, M ^{[1
]}

SAMUEL, S ^{[1
]}

LANGE, LA ^{[1
]}

WANG, C ^{[1
]}

CURPEN, GD ^{[1
]}

SAVANI, RC ^{[1
]}

GREENBERG, AH ^{[1
]}

TURLEY, EA ^{[1
]}

机构：

[1] UNIV MANITOBA, DEPT PEDIAT, WINNIPEG, MB R3E 0V9, CANADA

来源：

CELL | 1995年 / 82卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0092-8674(95)90048-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Overexpression of the RHAMM gene by transfection into fibroblasts is transforming and causes spontaneous metastases in the lung. H-ras-transformed fibrosarcomas transfected with a dominant suppressor mutant of RHAMM exhibit a so-called revertant phenotype end are completely nontumorigenic and nonmetastatic. Conversely, fibroblasts stably expressing low levels of RHAMM as a result of antisense transfection are resistant to res transformation. Collectively, these results indicate that RHAMM acts downstream of res. The loss of functional RHAMM ablates signaling within focal adhesions, in particular changes in focal adhesion kinase phosphorylation, and as a result these focal adhesions are unable to turn over in response to hyaluronan. These results provide evidence of the oncogenic potential of a novel extracellular matrix receptor and establish a functional link between transformation by ras and signaling within focal adhesions that are required for transformation by this oncogene.

引用

页码：19 / 28

页数：10

共 62 条

[11] TRANSFORMATION BY ONCOGENES ENCODING PROTEIN-KINASES INDUCES THE METASTATIC PHENOTYPE [J].

EGAN, SE ;

WRIGHT, JA ;

JAROLIM, L ;

YANAGIHARA, K ;

BASSIN, RH ;

GREENBERG, AH .

SCIENCE, 1987, 238 (4824) :202-205

[12]

ENTWISTLE J, 1995, IN PRESS GENE

[13] DOMINANT NEGATIVE MUTATION IN CELL-SURFACE BETA-1,4-GALACTOSYLTRANSFERASE INHIBITS CELL-CELL AND CELL MATRIX INTERACTIONS [J].

EVANS, SC ;

LOPEZ, LC ;

SHUR, BD .

JOURNAL OF CELL BIOLOGY, 1993, 120 (04) :1045-1057

[14] SUPPRESSION OF VINCULIN EXPRESSION BY ANTISENSE TRANSFECTION CONFERS CHANGES IN CELL MORPHOLOGY, MOTILITY, AND ANCHORAGE-DEPENDENT GROWTH OF 3T3-CELLS [J].

FERNANDEZ, JLR ;

GEIGER, B ;

SALOMON, D ;

BENZEEV, A .

JOURNAL OF CELL BIOLOGY, 1993, 122 (06) :1285-1294

[15]

FRASER R, 1993, FASEB J, V6, P2397

[16] ELEVATED LEVELS OF THE ALPHA-5-BETA-1-FIBRONECTIN RECEPTOR SUPPRESS THE TRANSFORMED PHENOTYPE OF CHINESE HAMSTER OVARY CELLS [J].

GIANCOTTI, FG ;

RUOSLAHTI, E .

CELL, 1990, 60 (05) :849-859

[17] A HUMAN BETA-ACTIN EXPRESSION VECTOR SYSTEM DIRECTS HIGH-LEVEL ACCUMULATION OF ANTISENSE TRANSCRIPTS [J].

GUNNING, P ;

LEAVITT, J ;

MUSCAT, G ;

NG, SY ;

KEDES, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (14) :4831-4835

[18]

Gunthert U, 1993, Curr Top Microbiol Immunol, V184, P47

[19] A NEW VARIANT OF GLYCOPROTEIN CD44 CONFERS METASTATIC POTENTIAL TO RAT CARCINOMA-CELLS [J].

GUNTHERT, U ;

HOFMANN, M ;

RUDY, W ;

REBER, S ;

ZOLLER, M ;

HAUSSMANN, I ;

MATZKU, S ;

WENZEL, A ;

PONTA, H ;

HERRLICH, P .

CELL, 1991, 65 (01) :13-24

[20] HYALURONAN AND THE HYALURONAN RECEPTOR RHAMM PROMOTE FOCAL ADHESION TURNOVER AND TRANSIENT TYROSINE KINASE-ACTIVITY [J].

HALL, CL ;

WANG, C ;

LANGE, LA ;

TURLEY, EA .

JOURNAL OF CELL BIOLOGY, 1994, 126 (02) :575-588

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