THE EFFECT OF SUPERHELICAL DENSITY ON THE YIELD OF SINGLE-STRAND BREAKS IN GAMMA-IRRADIATED PLASMID DNA

被引：31

作者：

MILLIGAN, JR

ARNOLD, AD

WARD, JF

机构：

[1] Department of Radiology, University of California, San Diego

来源：

RADIATION RESEARCH | 1992年 / 132卷 / 01期

关键词：

D O I：

10.2307/3578335

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Using agarose gel electrophoresis, the formation of DNA single-strand breaks (SSBs) by 137Cs γ irradiation was quantified in negatively supercoiled topological isomers of plasmid pUC18. The G value for SSB formation falls slightly from 1 x 108 to 8 x 10-9 SSB Gy-1 Da-1 as the superhelical density varies from 0.00 to -0.08. This result is not in agreement with recent observations by others which suggest that increasing the negative superhelical density of plasmid DNA increases its sensitivity to X irradiation.

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页码：69 / 73

页数：5

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