THE DIRECT DETERMINATION OF PROTEIN-CONCENTRATION FOR PROTEINS IMMOBILIZED ON POLYSTYRENE MICROSPHERES

被引：29

作者：

BASINSKA, T ^{[1
]}

SLOMKOWSKI, S ^{[1
]}

机构：

[1] POLISH ACAD SCI,CTR MOLEC & MACROMOLEC STUDIES,SIENKIEWICZA 112,PL-90363 LODZ,POLAND

来源：

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION | 1991年 / 3卷 / 02期

关键词：

DETERMINATION OF IMMOBILIZED PROTEINS; HUMAN SERUM ALBUMIN; IMMUNOGLOBULINS; POLYSTYRENE MICROSPHERES;

D O I：

10.1163/156856291X00223

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this paper we show how the Lowry method, designed for the determination of proteins in solution, can be used for the determination of proteins immobilized on the surface of microspheres (for protein concentrations higher than 10-mu-g/ml). Measurements were made for human serum albumin (HSA) and for immunoglobulins [rabbit immunoglobulins-antibodies against human fibrinogen (IgGF) and against fragment D of human fibrinogen (IgGFgD)] immobilized on the surface of polystyrene microspheres.

引用

页码：115 / 125

页数：11

共 26 条

[1] EFFECT OF PH ON THE ADSORPTION OF IMMUNOGLOBULIN-G ON ANIONIC POLY(VINYLTOLUENE) MODEL LATEX-PARTICLES
BAGCHI, P
BIRNBAUM, SM
[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1981, 83 (02) : 460 - 478
[2] THE IMMOBILIZATION OF ENZYMES ONTO HYDROLYZED POLYETHYLENE-G-CO-2-HEMA
BEDDOWS, CG
GIL, MH
GUTHRIE, JT
[J]. JOURNAL OF APPLIED POLYMER SCIENCE, 1988, 35 (01) : 135 - 144
[3] CHANGES IN ADSORBED FIBRINOGEN AND ALBUMIN INTERACTIONS WITH POLYMERS INDICATED BY DECREASES IN DETERGENT ELUTABILITY
BOHNERT, JL
HORBETT, TA
[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1986, 111 (02) : 363 - 378
[4] KINETIC STUDIES ON SOLID-SUPPORTED BETA-GALACTOSIDASE
BUNTING, PS
LAIDLER, KJ
[J]. BIOCHEMISTRY, 1972, 11 (24) : 4477 - &
[5] COLVIN M, 1988, MICROSPHERES MED BIO, P1
[6] COUVREUR P, 1986, POLYM NANOPARTICLES, P21
[7] Davis SS, 1986, POLYM NANOPARTICLES, P175
[8] ESKAMANI A, 1974, ANAL BIOCHEM, V57, P412
[9] Folin O, 1927, J BIOL CHEM, V73, P627
[10] Gabel D, 1976, Methods Enzymol, V44, P383

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