DOG LYMPHOCYTE-CULTURES FACILITATE THE ISOLATION AND GROWTH OF VIRULENT CANINE-DISTEMPER VIRUS

被引：43

作者：

APPEL, MJG

PEARCEKELLING, S

SUMMERS, BA

机构：

[1] James A. Baker Institute for Animal Health, Department of Microbiology, Immunology and Parasitology, Ithaca, NY

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 1992年 / 4卷 / 03期

关键词：

D O I：

10.1177/104063879200400306

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Optimal conditions for the isolation and growth of virulent canine distemper virus (CDV) in canine thymic and peripheral blood lymphocyte cultures were determined. Peak virus titers were seen from 3 to 6 days postinoculation of lymphocytes and depended on the multiplicity of infection. Dog lymphocytes were at least as susceptible as canine macrophages to infection with virulent CDV. Virus replication in lymphocytes resulted in higher virus titers than in dog lung macrophages. Peripheral blood lymphocytes (PBL) from CDV-immune dogs were as susceptible to CDV as were PBL from susceptible dogs.

引用

页码：258 / 263

页数：6