IDENTIFICATION OF A WEAK PROMOTER FOR THE DAM GENE OF ESCHERICHIA-COLI

被引：8
作者：
WU, TH
GRELLAND, E
BOYE, E
MARINUS, MG
机构：
[1] UNIV MASSACHUSETTS, SCH MED, DEPT PHARMACOL, 55 LAKE AVE, WORCESTER, MA 01655 USA
[2] INST CANC RES, DEPT BIOPHYS, OSLO, NORWAY
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1131卷 / 01期
关键词：
DNA METHYLATION; GENE EXPRESSION; PROMOTER; DAM GENE; TRANSCRIPTION; (ESCHERICHIA-COLI);
D O I：
10.1016/0167-4781(92)90097-J
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have used a combination of techniques to identify a weak promoter located about 70 nucleotides before the start site of translation of thc Escherichia coli dam gene which encodes a DNA methyltransferase. Thc promoter activity was identified by the use of lacZ fusions to fragments containing different lengths of upstream DNA. In vitro run-off transcription and primer extension determinations revealed transcription initiation sites at either 69 or 73 nucleotides prior to thc ATG of the dam coding sequence. No ribosome binding sequence was present close to thc ATG codon suggesting that thc transcript may be inefficiently translated.
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页码：47 / 52
页数：6
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