IDENTIFICATION OF A WEAK PROMOTER FOR THE DAM GENE OF ESCHERICHIA-COLI

被引：8
作者：
WU, TH
GRELLAND, E
BOYE, E
MARINUS, MG
机构：
[1] UNIV MASSACHUSETTS, SCH MED, DEPT PHARMACOL, 55 LAKE AVE, WORCESTER, MA 01655 USA
[2] INST CANC RES, DEPT BIOPHYS, OSLO, NORWAY
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1131卷 / 01期
关键词：
DNA METHYLATION; GENE EXPRESSION; PROMOTER; DAM GENE; TRANSCRIPTION; (ESCHERICHIA-COLI);
D O I：
10.1016/0167-4781(92)90097-J
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have used a combination of techniques to identify a weak promoter located about 70 nucleotides before the start site of translation of thc Escherichia coli dam gene which encodes a DNA methyltransferase. Thc promoter activity was identified by the use of lacZ fusions to fragments containing different lengths of upstream DNA. In vitro run-off transcription and primer extension determinations revealed transcription initiation sites at either 69 or 73 nucleotides prior to thc ATG of the dam coding sequence. No ribosome binding sequence was present close to thc ATG codon suggesting that thc transcript may be inefficiently translated.
引用
收藏
页码：47 / 52
页数：6
相关论文
共 31 条
[1]  
AIBA H, 1981, J BIOL CHEM, V256, P1905
[2]   EXPRESSION OF A DNA METHYLATION (DAM) GENE IN ESCHERICHIA-COLI K-12 [J].
ARRAJ, JA ;
WU, TH ;
MARINUS, MG .
CURRENT MICROBIOLOGY, 1990, 20 (02) :133-136
[3]   PHENOTYPIC REVERSAL IN DAM MUTANTS OF ESCHERICHIA-COLI K-12 BY A RECOMBINANT PLASMID CONTAINING THE DAM+ GENE [J].
ARRAJ, JA ;
MARINUS, MG .
JOURNAL OF BACTERIOLOGY, 1983, 153 (01) :562-565
[4]   AUTO-REGULATION OF THE DNAA GENE OF ESCHERICHIA-COLI-K12 [J].
ATLUNG, T ;
CLAUSEN, ES ;
HANSEN, FG .
MOLECULAR & GENERAL GENETICS, 1985, 200 (03) :442-450
[5]   LINKAGE MAP OF ESCHERICHIA-COLI K-12, EDITION-8 [J].
BACHMANN, BJ .
MICROBIOLOGICAL REVIEWS, 1990, 54 (02) :130-197
[6]   THE GREAT GATC - DNA METHYLATION IN ESCHERICHIA-COLI [J].
BARRAS, F ;
MARINUS, MG .
TRENDS IN GENETICS, 1989, 5 (05) :139-143
[7]   THE ROLE OF DAM METHYLTRANSFERASE IN THE CONTROL OF DNA-REPLICATION IN ESCHERICHIA-COLI [J].
BOYE, E ;
LOBNEROLESEN, A .
CELL, 1990, 62 (05) :981-989
[8]   THE ISOLATION AND CHARACTERIZATION OF THE ESCHERICHIA-COLI DNA ADENINE METHYLASE (DAM) GENE [J].
BROOKS, JE ;
BLUMENTHAL, RM ;
GINGERAS, TR .
NUCLEIC ACIDS RESEARCH, 1983, 11 (03) :837-851
[9]   ESCHERICHIA-COLI ORIC AND THE DNAA GENE PROMOTER ARE SEQUESTERED FROM DAM METHYLTRANSFERASE FOLLOWING THE PASSAGE OF THE CHROMOSOMAL REPLICATION FORK [J].
CAMPBELL, JL ;
KLECKNER, N .
CELL, 1990, 62 (05) :967-979
[10]   MUTATIONS PRODUCED BY DNA POLYMERASE-III HOLOENZYME OF ESCHERICHIA-COLI AFTER INVITRO SYNTHESIS IN THE ABSENCE OF SINGLE-STRAND BINDING-PROTEIN [J].
CARRAWAY, M ;
REWINSKI, C ;
MARINUS, MG .
MOLECULAR MICROBIOLOGY, 1990, 4 (10) :1645-1652
1234