Streptomyces apocyni sp. nov., an endogenous actinomycete isolated from Apocynum venetum

A novel actinomycete, designated strain TRM 66233(T). was isolated from Apocynum venetum L. collected from the Xinjiang Uygur Autonomous Region of China and characterized using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain TRM 66233(T) with the genus Streptomyces. Strain TRM 66233(T) showed a high similarity value to Streptomyces bikiniensis NRRL B-1049(T) (98.07%) based on the 16S rRNA gene phylogenetic tree. The whole-cell sugar pattern of TRM 66233(T) consisted of glucose, galactose, mannose and ribose. The predominant menaquinones were MK-9(H-2), MK-9(H-6), MK-9(H-8) and MK-9(H-10). The polar lipids were diphosphatidylglycera phosphatidylethanolamine, phosphatidylinosi- tol and four unidentified lipids. The major fatty acids were iso-C-15(.0), anteiso-C-15.0, iso-C-1(6).0 and iso-C-17.0. The G+C content of the DNA was 70.35 mol%. The DNA-DNA relatedness and average nucleotide identity values as well as evolutionary distances based on multilocus (atpD, gyrB, recA, rpoB and trpB) sequences between strain TRM 66233(T) and closely related type strains were significantly lower than the recommended threshold values. The whole-genome average nucleotide identity and digital DNA-DNA hybridization values between strain TRM 66233(T) and S. bikiniensis NRRL B-1049(T) were 78.86 and 23.2%, respectively. On the basis of evidence from this polyphasic study, strain TRM 66233(T) should represent a novel species of the genus Streptomyces, for which the name Streptomyces apocyni sp. nov. is proposed. The type strain is TRM 66233(T) (=CCTCC AA 2019056(T) = LMG 31559(T).