P25(RUM1) ORDERS S-PHASE AND MITOSIS BY ACTING AS AN INHIBITOR OF THE P34(CDC2) MITOTIC KINASE

被引：174
作者：
CORREABORDES, J
NURSE, P
机构：
[1] Imperial Cancer Research Fund Lincoln's Inn Fields, London
来源：
CELL | 1995年 / 83卷 / 06期
关键词：
D O I：
10.1016/0092-8674(95)90215-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
p25(rum1) from the fission yeast S. pombe is shown to act as a specific in vitro inhibitor of the p34(cdc2)/p56(cdc13) mitotic kinase. It is also shown that early G1 cells contain p25(rum1), which associates with and inhibits the mitotic kinase, and maintains p56(cdc13) miotic B cyclin at a low level, ensuring that these cells do not undergo a premature lethal entry into mitosis. A high level of p25(rum1) in G2 cells inhibits the p34(cdc2)/p56(cdc13) kinase that removes the block preventing a further S phase and leads to repeated rounds of DNA replication. Thus, the cyclin-dependent kinase inhibitor p25(rum1), acting on the p34(cdc2) mitotic kinase, plays an important role in ensuring the correct sequence of S phase and mitosis during the cell cycle.
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页码：1001 / 1009
页数：9
相关论文
共 45 条
[11]  
FISHER DL, 1996, IN PRESS EMBO J
[12]   TYROSINE PHOSPHORYLATION OF THE FISSION YEAST CDC2+ PROTEIN-KINASE REGULATES ENTRY INTO MITOSIS [J].
GOULD, KL ;
NURSE, P .
NATURE, 1989, 342 (6245) :39-45
[13]   A PRE-START CHECKPOINT PREVENTING MITOSIS IN FISSION YEAST ACTS INDEPENDENTLY OF P34(CDC2) TYROSINE PHOSPHORYLATION [J].
HAYLES, J ;
NURSE, P .
EMBO JOURNAL, 1995, 14 (12) :2760-2771
[14]   TEMPORAL-ORDER OF S-PHASE AND MITOSIS IN FISSION YEAST IS DETERMINED BY THE STATE OF THE P34(CDC2) MITOTIC B-CYCLIN COMPLEX [J].
HAYLES, J ;
FISHER, D ;
WOOLLARD, A ;
NURSE, P .
CELL, 1994, 78 (05) :813-822
[15]   A TEMPERATURE-SENSITIVE MUTATION OF THE SCHIZOSACCHAROMYCES-POMBE GENE NUC2+THAT ENCODES A NUCLEAR SCAFFOLD-LIKE PROTEIN BLOCKS SPINDLE ELONGATION IN MITOTIC ANAPHASE [J].
HIRANO, T ;
HIRAOKA, Y ;
YANAGIDA, M .
JOURNAL OF CELL BIOLOGY, 1988, 106 (04) :1171-1183
[16]   GENES INVOLVED IN SISTER-CHROMATID SEPARATION ARE NEEDED FOR B-TYPE CYCLIN PROTEOLYSIS IN BUDDING YEAST [J].
IRNIGER, S ;
PIATTI, S ;
MICHAELIS, C ;
NASMYTH, K .
CELL, 1995, 81 (02) :269-278
[17]   THE FISSION YEAST CDC18+ GENE-PRODUCT COUPLES S-PHASE TO START AND MITOSIS [J].
KELLY, TJ ;
MARTIN, GS ;
FORSBURG, SL ;
STEPHEN, RJ ;
RUSSO, A ;
NURSE, P .
CELL, 1993, 74 (02) :371-382
[18]   A 20S COMPLEX CONTAINING CDC27 AND CDC16 CATALYZES THE MITOSIS-SPECIFIC CONJUGATION OF UBIQUITIN TO CYCLIN-B [J].
KING, RW ;
PETERS, JM ;
TUGENDREICH, S ;
ROLFE, M ;
HIETER, P ;
KIRSCHNER, MW .
CELL, 1995, 81 (02) :279-288
[19]   MITOSIS IN TRANSITION [J].
KING, RW ;
JACKSON, PK ;
KIRSCHNER, MW .
CELL, 1994, 79 (04) :563-571
[20]   DIFFERENTIAL PHOSPHORYLATION OF VERTEBRATE P34CDC2 KINASE AT THE G1/S AND G2/M TRANSITIONS OF THE CELL-CYCLE - IDENTIFICATION OF MAJOR PHOSPHORYLATION SITES [J].
KREK, W ;
NIGG, EA .
EMBO JOURNAL, 1991, 10 (02) :305-316
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