PARA-AMINOBENZOATE SYNTHASE GENE OF SACCHAROMYCES-CEREVISIAE ENCODES A BIFUNCTIONAL ENZYME

被引：36
作者：
EDMAN, JC
GOLDSTEIN, AL
ERBE, JG
机构：
[1] Department of Laboratory Medicine, University of California, San Francisco, California
[2] Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire
来源：
YEAST | 1993年 / 9卷 / 06期
关键词：
CHROMOSOME-XIV; SACCHAROMYCES-CEREVISIAE; PABA SYNTHASE; DNA SEQUENCING;
D O I：
10.1002/yea.320090613
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Saccharomyces cerevisiae gene for para-aminobenzoate (PABA) synthase has been identified based upon its ability to confer sulfonamide resistance when present on a multicopy episomal vector. The 3840 bp DNA sequence fragment reported here contains a 2199 bp open reading frame encoding a 733 amino acid protein with similarity to the two components of PABA synthase described for prokaryotes (Escherichia coli PabA and PabB), suggesting that PABA synthase is bifunctional in yeast. The cloned sequence was confirmed to be PABA synthase by gene disruption. Chromosome gel analysis places the gene for PABA synthase on chromosome XIV.
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页码：669 / 675
页数：7
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