BIACORE AS A TOOL IN ANTIBODY ENGINEERING

被引：54
作者：
MALMBORG, AC [1 ]
BORREBAECK, CAK [1 ]
机构：
[1] LUND UNIV,DEPT IMMUNOTECHNOL,S-22007 LUND,SWEDEN
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1995年 / 183卷 / 01期
关键词：
BIOSENSOR; PHAGE DISPLAY; KINETIC SELECTION; RECOMBINANT ANTIBODY; ANTIBODY LIBRARY;
D O I：
10.1016/0022-1759(95)00018-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The BIAcore biosensor provides a simple and rapid approach for analysing recombinant antibodies and phage displayed antibody libraries. In this review we describe the application of the biosensor in the screening of recombinant antibody fragments, kinetic selection of phage displayed antibodies, characterization and epitope mapping of monoclonal antibodies and their fragments.
引用
收藏
页码：7 / 13
页数：7
相关论文
共 40 条
[1]   HIGH-AFFINITY SELF-REACTIVE HUMAN-ANTIBODIES BY DESIGN AND SELECTION - TARGETING THE INTEGRIN LIGAND-BINDING SITE [J].
BARBAS, CF ;
LANGUINO, LR ;
SMITH, JW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (21) :10003-10007
[2]   KINETIC-ANALYSIS OF RECOMBINANT ANTIBODY-ANTIGEN INTERACTIONS - RELATION BETWEEN STRUCTURAL DOMAINS AND ANTIGEN-BINDING [J].
BORREBAECK, CAK ;
MALMBORG, AC ;
FUREBRING, C ;
MICHAELSSON, A ;
WARD, S ;
DANIELSSON, L ;
OHLIN, M .
BIO-TECHNOLOGY, 1992, 10 (06) :697-698
[3]   A LARGE ARRAY OF HUMAN MONOCLONAL-ANTIBODIES TO TYPE-1 HUMAN-IMMUNODEFICIENCY-VIRUS FROM COMBINATORIAL LIBRARIES OF ASYMPTOMATIC SEROPOSITIVE INDIVIDUALS [J].
BURTON, DR ;
BARBAS, CF ;
PERSSON, MAA ;
KOENIG, S ;
CHANOCK, RM ;
LERNER, RA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (22) :10134-10137
[4]   VARIABLE DOMAIN-IDENTICAL ANTIBODIES EXHIBIT IGG SUBCLASS-RELATED DIFFERENCES IN AFFINITY AND KINETIC CONSTANTS AS DETERMINED BY SURFACE-PLASMON RESONANCE [J].
COOPER, LJN ;
ROBERTSON, D ;
GRANZOW, R ;
GREENSPAN, NS .
MOLECULAR IMMUNOLOGY, 1994, 31 (08) :577-584
[5]  
DENG SJ, 1994, J BIOL CHEM, V269, P9533
[6]  
DUENAS M, 1994, IN PRESS BIOTECHNOLO
[7]  
DUENAS M, 1994, UNPUB PHAGE DISPLAYE
[8]  
Fagerstam L G, 1990, J Mol Recognit, V3, P208, DOI 10.1002/jmr.300030507
[9]   ANTIGEN-ANTIBODY BINDING AND MASS-TRANSPORT BY CONVECTION AND DIFFUSION TO A SURFACE - A 2-DIMENSIONAL COMPUTER-MODEL OF BINDING AND DISSOCIATION KINETICS [J].
GLASER, RW .
ANALYTICAL BIOCHEMISTRY, 1993, 213 (01) :152-161
[10]  
GLASER SM, 1992, J IMMUNOL, V149, P3903
1234