SEPARATION OF COUMARINS BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

被引：23

作者：

CHEN, CT ^{[1
]}

SHEU, SJ ^{[1
]}

机构：

[1] NATL TAIWAN NORMAL UNIV, DEPT CHEM, TAIPEI, TAIWAN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 710卷 / 02期

关键词：

D O I：

10.1016/0021-9673(95)00509-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nine coumarins were successfully separated simultaneously using micellar electrokinetic capillary chromatography with 4-hydroxybenzoic acid as an internal standard. A carrier composed of buffer solution (20 mM sodium dodecyl sulfate-15 mM sodium borate-15 mM sodium dihydrogenphosphate)-acetonitrile (24:1) was found to be the most suitable electrolyte for this separation. The analysis time (22 min) was shorter than that using high-performance liquid chromatography (47 min). Contents of coumarins in the crude drug of Angelicae Tuhou Radix could be easily determined by the proposed method.

引用

页码：323 / 329

页数：7

共 23 条

[1]

BABA K, 1982, CHEM PHARM BULL, V30, P2025

[2]

CHEN S, 1982, YAOXUE TONGBAO, V17, P120

[3]

Chen S Q, 1982, Yao Xue Xue Bao, V17, P392

[4]

GAO H, 1992, YAOWU FENXI ZAZHI, V12, P221

[5] ON COUMARINS FROM THE FRUITS OF ANGELICA-PUBESCENS MAXIM [J].

HATA, K ;

NISHINO, T ;

HIRAI, Y ;

WADA, Y ;

KOZAWA, M .

YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 1981, 101 (01) :67-71

[6]

HATA K, 1971, CHEM PHARM BULL, V19, P640

[7]

HATA K, 1965, TETRAHEDRON LETT, P4557

[8]

Hata K, 1967, Yakugaku Zasshi, V87, P210

[9]

Hata K., 1957, YAKUGAKU ZASSHI, V77, P937, DOI [10.1248/yakushi1947.77.9_937, DOI 10.1248/YAKUSHI1947.77.9_937]

[10]

HATA KIYOSHI, 1968, YAKUGAKU ZASSHI [J PHARM SOC JAP], V88, P293

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