PROTEOLYTIC ACTIVITY IN EXTRACTS OF INVASIVE CERVICAL-CARCINOMA AND PRECURSOR LESIONS

被引:12
作者
DANERINAVARRO, A
DELTOROARREOLA, S
BRAVOCUELLAR, A
CABRERA, N
ORBACHARBOUYS, S
PEREZMONTFORT, R
机构
[1] UNIV GUADALAJARA,GUADALAJARA 44280,JALISCO,MEXICO
[2] IMSS,CTR INVEST BIOMED OCCIDENTE,GUADALAJARA,JALISCO,MEXICO
[3] UNIV NACL AUTONOMA MEXICO,INST FISIOL CELULAR,DEPT MICROBIOL,MEXICO CITY 04510,DF,MEXICO
[4] HOP SUISSE PARIS,INST CANC & IMMUNOL,ISSY MOULINEAUX,FRANCE
关键词
CERVICAL CARCINOMA; PROTEINASES; METALLOPROTEINASES;
D O I
10.1016/0753-3322(96)82648-5
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
In this preliminary report, we showed that proteolytic activity of extracts from 85 cervical samples of patients with normal cervix, low and high-grade squamous intra-epithelial lesions and invasive carcinoma, increased according to the natural history of the cervical cancer when measured with three different substrates. Inhibitor assays for four different catalytic classes of endopeptidases indicated that the predominant catalytic class in extracts of all groups was that of metalloproteinases. Substrate gel electrophoresis revealed that invasive carcinoma extracts had two bands with proteolytic activity (with M(r) of 72 and 52 kDa) which were not present in normal tissue or biopsies with precursor lesions. Immunological and molecular characterization of these bands may provide information relevant to cervical cancer biology and clinical applications.
引用
收藏
页码:304 / 310
页数:7
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