PURIFICATION AND CHARACTERIZATION OF DISTINCT TYPE OF MANNOSE-SENSITIVE FIMBRIAE FROM SALMONELLA-TYPHIMURIUM

被引：0

作者：

GHOSH, S ^{[1
]}

MITTAL, A ^{[1
]}

GANGULY, NK ^{[1
]}

机构：

[1] POSTGRAD INST MED EDUC & RES,DEPT EXPTL MED,CHANDIGARH 160012,INDIA

来源：

FEMS MICROBIOLOGY LETTERS | 1994年 / 115卷 / 2-3期

关键词：

SALMONELLA TYPHIMURIUM; FIMBRIAE; HEMAGGLUTINATION;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The fimbriae (E(4)) of a virulent strain of Salmonella typhimurium were purified by ion exchange chromatography in an FPLC system. They had a channelled appearance under transmission electron microscope and showed a major structural subunit of 17-kDa on sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The purified fimbriae were found to agglutinate guinea pig erythrocytes, but this effect was inhibited in presence of D-mannose. Immune sera raised against the Mono-Q purified fimbriae (E(4)) showed cross-reactivity with the type-1 fimbriae (F-1) composed of 21-kDa fimbrin subunit, purified by a different method from the same strain.

引用

页码：229 / 234

页数：6

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