GLYCOPROTEIN GLYCOSYLTRANSFERASE ACTIVITIES IN SERUM IN ALCOHOL-ABUSING PATIENTS AND HEALTHY CONTROLS

The activities of glycoprotein sialyl-, galactosyl- and N-acetylglucosaminyltransferase in serum were examined in 14 alcoholic patients with a median BAC of 65 mmol/l and in 14 healthy age- and sex-matched controls. The median alcohol intake of the patients was 250 g/day for 1-3 weeks. All had elevated levels of carbohydrate-deficient transferrin in serum and normal or near normal liver function tests. The activities of galactosyl- and N-acetylglucosaminyltransferase at subsaturated substrate concentration were significantly reduced in the patients. V(max) for all three enzymes was 35-55% lower in the patients than in the controls. Ethanol up to 500 mmol/l had no effect on these enzymes in serum. Acetaldehyde up to 200-mu-mol/l produced a significant dose-dependent inhibition of all three glycosyltransferases ranging from 19 to 44% at 200-mu-mol/l. Pyridoxal-5'-phosphate had a similar inhibitory effect on all enzyme activities suggesting the presence of lysine in the substrate or acceptor sites, or in the glycoprotein acceptors. The latter finding provides a possible molecular basis for acetaldehyde to modify glycosyl transfer. The results indicate that in man, alcohol abuse is connected with reduced activities of several glycosyltransferases in serum, which may reflect an important mechanism behind the carbohydrate deficiencies in secretory as well as membrane-bound glyconjugates in alcoholic patients.