DIRECT DETERMINATION OF FREE FATTY-ACID TRANSPORT ACROSS THE ADIPOCYTE PLASMA-MEMBRANE USING QUANTITATIVE FLUORESCENCE MICROSCOPY

Movement of free fatty acids (FFA) across the plasma membrane has been directly measured for the first time, using fluorescent FFA analogs and quantitative fluorescence microscopy. The rate of short chain FFA (less-than-or-equal-to 12 carbons) transport from the extracellular medium into intracellular lipid droplets of 3T3F442A adipocytes was more than 40-fold faster than long chain FFA (16 and 18 carbons). The membrane-impermeable amino reagent 4,4'-diisothiocyanostilbene-2,2'-disulfonate, inhibited greater-than-or-equal-to 50% of the long chain FFA transport but had no effect on short chain FFA transport. Oleic acid (2-mu-M) inhibited 90% of the fluorescent oleate transport but had no effect on the 11-carbon analog. These results indicate that a large fraction of long chain FFA uptake is mediated by a plasma membrane protein (s).