MOLECULAR-GENETIC ANALYSIS OF BETA-TOXIN OF CLOSTRIDIUM-PERFRINGENS REVEALS SEQUENCE HOMOLOGY WITH ALPHA-TOXIN, GAMMA-TOXIN, AND LEUKOCIDIN OF STAPHYLOCOCCUS-AUREUS

被引：123
|
作者：
HUNTER, SEC
BROWN, JE
OYSTON, PCF
SAKURAI, J
TITBALL, RW
机构：
[1] CHEM & BIOL DEF ESTAB,SALISBURY SP4 0JQ,WILTS,ENGLAND
[2] USA,MED RES INST INFECT DIS,FT DETRICK,FREDERICK,MD 21702
[3] TOKUSHIMA BUNRI UNIV,FAC PHARMACEUT SCI,TOKUSHIMA 770,JAPAN
来源：
INFECTION AND IMMUNITY | 1993年 / 61卷 / 09期
关键词：
D O I：
10.1128/IAI.61.9.3958-3965.1993
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Oligonucleotide probes designed on the basis of the N-terminal sequence of Clostridium perfringens beta-toxin were used to isolate the encoding gene (cpb). The nucleotide sequence of cpb was determined, and on the basis of DNA hybridization experiments it was shown that the gene is found only in type B and C strains of C. perfringens. The deduced amino acid sequence of the beta-toxin revealed homology with the alpha-toxin, gamma-toxin, and leukocidin of Staphylococcus aureus. The beta-toxin purified from C. perfringens appeared to exist in monomeric and multimeric forms. Recombinant beta-toxin, produced in Escherichia coli, appeared to be mainly in the multimeric form.
引用
收藏
页码：3958 / 3965
页数：8
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