BRUCELLA GROUP-3 OUTER-MEMBRANE PROTEINS CONTAIN A HEAT-MODIFIABLE PROTEIN

被引：0

作者：

GAMAZO, C ^{[1
]}

VITAS, AI ^{[1
]}

MORIYON, I ^{[1
]}

LOPEZGONI, I ^{[1
]}

DIAZ, R ^{[1
]}

机构：

[1] UNIV NAVARRA CLIN,DEPT MICROBIOL,PAMPLONA,SPAIN

来源：

FEMS MICROBIOLOGY LETTERS | 1993年 / 112卷 / 02期

关键词：

BRUCELLA; OMPA; OUTER MEMBRANE BLEBS;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Brucella melitensis and B. ovis outer membrane blebs contained a protein displaying a temperature-dependent molecular mass upshift from 25 kDa to 30 kDa. A fraction of the protein tightly bound to LPS did not show the molecular mass upshift which was also blocked by exposure of the protein to Zwittergent 314. The B. melitensis heat-modifiable protein and Escherichia coli OmpA shared antigenic determinants. These data indicate that the Brucella group 3 outer membrane proteins belonged to the OmpA family of proteins.

引用

收藏

页码：141 / 146

页数：6

相关论文

共 20 条

[1] MAJOR HEAT-MODIFIABLE OUTER-MEMBRANE PROTEIN IN GRAM-NEGATIVE BACTERIA - COMPARISON WITH THE OMPA PROTEIN OF ESCHERICHIA-COLI [J].

BEHER, MG ;

SCHNAITMAN, CA ;

PUGSLEY, AP .

JOURNAL OF BACTERIOLOGY, 1980, 143 (02) :906-913

[2] PORINS OF BRUCELLA SPECIES [J].

DOUGLAS, JT ;

ROSENBERG, EY ;

NIKAIDO, H ;

VERSTREATE, DR ;

WINTER, AJ .

INFECTION AND IMMUNITY, 1984, 44 (01) :16-21

[3] ELECTROPHORETIC ANALYSIS OF MAJOR POLYPEPTIDES OF HUMAN ERYTHROCYTE MEMBRANE [J].

FAIRBANKS, G ;

STECK, TL ;

WALLACH, DFH .

BIOCHEMISTRY, 1971, 10 (13) :2606-+

[4] COMPARATIVE ANALYSES OF PROTEINS EXTRACTED BY HOT SALINE OR RELEASED SPONTANEOUSLY INTO OUTER-MEMBRANE BLEBS FROM FIELD STRAINS OF BRUCELLA-OVIS AND BRUCELLA-MELITENSIS [J].

GAMAZO, C ;

WINTER, AJ ;

MORIYON, I ;

RIEZUBOJ, JI ;

BLASCO, JM ;

DIAZ, R .

INFECTION AND IMMUNITY, 1989, 57 (05) :1419-1426

[5] RELEASE OF OUTER-MEMBRANE FRAGMENTS BY EXPONENTIALLY GROWING BRUCELLA-MELITENSIS CELLS [J].

GAMAZO, C ;

MORIYON, I .

INFECTION AND IMMUNITY, 1987, 55 (03) :609-615

[6] THE DNA-SEQUENCE OF THE STRUCTURAL GENE OF GONOCOCCAL PROTEIN-III AND THE FLANKING REGION CONTAINING A REPETITIVE SEQUENCE - HOMOLOGY OF PROTEIN-III WITH ENTEROBACTERIAL OMPA PROTEINS [J].

GOTSCHLICH, EC ;

SEIFF, M ;

BLAKE, MS .

JOURNAL OF EXPERIMENTAL MEDICINE, 1987, 165 (02) :471-482

[7] OUTER-MEMBRANE OF PSEUDOMONAS-AERUGINOSA - HEAT AND 2-MERCAPTOETHANOL MODIFIABLE PROTEINS [J].

HANCOCK, REW ;

CAREY, AM .

JOURNAL OF BACTERIOLOGY, 1979, 140 (03) :902-910

[8] MAJOR PROTEINS OF ESCHERICHIA-COLI OUTER CELL-ENVELOPE MEMBRANE - PREPARATIVE ISOLATION OF ALL MAJOR MEMBRANE PROTEINS [J].

HINDENNACH, I ;

HENNING, U .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1975, 59 (01) :207-213

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10] MOLECULAR ARCHITECTURE AND FUNCTIONING OF THE OUTER-MEMBRANE OF ESCHERICHIA-COLI AND OTHER GRAM-NEGATIVE BACTERIA [J].

LUGTENBERG, B ;

VANALPHEN, L .

BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 737 (01) :51-115