MOLECULAR-CLONING AND DNA-SEQUENCE ANALYSIS OF CDNA-ENCODING CHICKEN HOMOLOG OF THE BCL-2-ONCOPROTEIN

被引：37

作者：

CAZALSHATEM, DL ^{[1
]}

LOUIE, DC ^{[1
]}

TANAKA, S ^{[1
]}

REED, JC ^{[1
]}

机构：

[1] UNIV PENN,DEPT PATHOL & LAB MED,PHILADELPHIA,PA 19104

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1132卷 / 01期

关键词：

BCL-2; ONCOPROTEIN; MITOCHONDRION; NUCLEOTIDE SEQUENCE; APOPTOSIS; PROGRAMMED CELL DEATH; (CHICKEN);

D O I：

10.1016/0167-4781(92)90064-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a 2228 bp cDNA clone encoding a chicken homologue of the human Bcl-2 oncoprotein by low-stringency hybridization screening of a lambda-gt10 cDNA library derived from a chicken B-cell lymphoma. DNA sequence analysis of this cDNA revealed an open reading frame predicting a polypeptide of 232 amino acids and an M(r) of 25 839. The predicted protein is highly homologous to the human (73%) and mouse (70%) Bcl-2 proteins, and contains a hydrophobic stretch of amino acids within its carboxyl-end (213-229) consistent with an integral membrane protein. Areas of very high sequence homology shared by all three Bcl-2 proteins at the NH2-terminus (amino acids 1-33) and within the last 150 amino acids of these proteins suggest the presence of at least two evolutionarily conserved domains within the family of Bcl-2 proteins that may be important either for their targeting to mitochondria or their ability to block programmed cell death.

引用

页码：109 / 113

页数：5

共 21 条

[1] PROSITE - A DICTIONARY OF SITES AND PATTERNS IN PROTEINS [J].

BAIROCH, A .

NUCLEIC ACIDS RESEARCH, 1991, 19 :2241-2245

[2]

FRASER CM, 1989, J BIOL CHEM, V264, P9266

[3]

FUJII J, 1989, J BIOL CHEM, V264, P12950

[4] ANALYSIS OF ACCURACY AND IMPLICATIONS OF SIMPLE METHODS FOR PREDICTING SECONDARY STRUCTURE OF GLOBULAR PROTEINS [J].

GARNIER, J ;

OSGUTHORPE, DJ ;

ROBSON, B .

JOURNAL OF MOLECULAR BIOLOGY, 1978, 120 (01) :97-120

[5] CLEAVAGE-SITE MOTIFS IN MITOCHONDRIAL TARGETING PEPTIDES [J].

GAVEL, Y ;

VONHEIJNE, G .

PROTEIN ENGINEERING, 1990, 4 (01) :33-37

[6] MITOCHONDRIAL PROTEIN CHARGE [J].

HARTMANN, C ;

CHRISTEN, P ;

JAUSSI, R .

NATURE, 1991, 352 (6338) :762-763

[7]

HIGGINS DG, 1989, COMPUT APPL BIOSCI, V5, P151

[8] BCL-2 IS AN INNER MITOCHONDRIAL-MEMBRANE PROTEIN THAT BLOCKS PROGRAMMED CELL-DEATH [J].

HOCKENBERY, D ;

NUNEZ, G ;

MILLIMAN, C ;

SCHREIBER, RD ;

KORSMEYER, SJ .

NATURE, 1990, 348 (6299) :334-336

[9] THE DETECTION AND CLASSIFICATION OF MEMBRANE-SPANNING PROTEINS [J].

KLEIN, P ;

KANEHISA, M ;

DELISI, C .

BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 815 (03) :468-476

[10]

KOZAK M, 1991, J BIOL CHEM, V266, P19867

← 1 2 3 →