ENDOGENOUS RETROVIRAL LTR DNA-SEQUENCES AS MARKERS FOR INDIVIDUAL HUMAN-CHROMOSOMES

被引：11

作者：

NAKAMURA, N ^{[1
]}

SUGINO, H ^{[1
]}

TAKAHARA, K ^{[1
]}

JIN, C ^{[1
]}

FUKUSHIGE, S ^{[1
]}

MATSUBARA, K ^{[1
]}

机构：

[1] OSAKA UNIV,INST MOLEC & CELLULAR BIOL,SUITA,OSAKA 565,JAPAN

来源：

CYTOGENETICS AND CELL GENETICS | 1991年 / 57卷 / 01期

关键词：

D O I：

10.1159/000133105

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The human genome carries multiple copies of sequences related to endogenous retroviral genomes that include long terminal repeat (LTR) sequences. We used the LTR of one such viral genome, called HERV-A, as a probe in Southern analysis to examine the distribution profiles of the hybridizing DNA in the genomes of twelve human X rodent hybrid cell lines carrying one or a few human chromosomes, and in the DNA samples prepared from six sorted, individual chromosomes. The HERV-A sequence was found to be widely distributed among different chromosomes and the Southern patterns for chromosomes 5, the X, and the Y, each obtained in duplicate from independently prepared cell lines or sorted chromosomes, were matched. Chromosome-specific Southern profiles can be used to monitor chromosomes in hybrid cells or to characterize chromosome aberrations, such as deletions.

引用

页码：18 / 22

页数：5

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