NOVEL ROUTE TO OLIGO(DEOXYRIBONUCLEOSIDE PHOSPHOROTHIOATES) - STEREOCONTROLLED SYNTHESIS OF P-CHIRAL OLIGO(DEOXYRIBONUCLEOSIDE PHOSPHOROTHIOATES)

被引：147

作者：

STEC, WJ

GRAJKOWSKI, A

KOZIOLKIEWICZ, M

UZNANSKI, B

机构：

[1] Polish Academy of Sciences, Centre of Molecular and Macromolecular Studies, Department of Bioorganic Chemistry, Lodz 90-363

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 21期

关键词：

D O I：

10.1093/nar/19.21.5883

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis and separation of diastereoisomerically pure 5'-O-DMT-nucleoside 3'-O-(2-thio-1,3,2-oxathiaphospholane) allows their use as synthons in DBU-catalyzed reaction with the 5'-hydroxyl function of solid-support-bound nucleoside moiety. Since this reaction is stereospecific (> 99%), this novel method allows preparation of oligo(nucleoside phosphorothioates) (1) with predetermined chirality at each P-chiral internucleotide phosphorothioate centre.

引用

页码：5883 / 5888

页数：6

共 23 条

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