EXPRESSION AND POSSIBLE FUNCTION OF GLUCOSE TRANSPORTER PROTEIN GLUT1 DURING PREIMPLANTATION MOUSE DEVELOPMENT FROM OOCYTES TO BLASTOCYSTS

被引：57
作者：
MORITA, Y
TSUTSUMI, O
HOSOYA, I
TAKETANI, Y
OKA, Y
KATO, T
机构：
[1] UNIV TOKYO,FAC MED,DEPT OBSTET & GYNECOL,BUNKYO KU,TOKYO 113,JAPAN
[2] UNIV TOKYO,FAC MED,DEPT INTERNAL MED 3,BUNKYO KU,TOKYO 113,JAPAN
[3] YOKOHAMA CITY UNIV,SCH MED,DEPT BIOCHEM,KANAZAWA KU,YOKOHAMA 236,JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 188卷 / 01期
关键词：
D O I：
10.1016/0006-291X(92)92342-U
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A micro-Western analysis method both sensitive and quantitative enough to analyze oocytes and embryos is developed. GLUT1 protein is present in mouse oocytes and preimplantation embryos and levels are increased by fertilization and with time in ensuing embryonic development; the levels were 20-fold greater in blastocysts than in unfertilized oocytes. Similar increases were observed in glucose uptake by oocytes and embryos, suggesting that they may depend on GLUT1 expression. These results suggest that GLUT1 expression may explain a switch in substrate preference of the embryo from pyruvate to glucose during preimplantation development. © 1992.
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页码：8 / 15
页数：8
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