COMPARATIVE-STUDY ON 16 ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR THE DETECTION OF ANTIBODIES TO BOVINE HERPESVIRUS-1 IN CATTLE

Sixteen commercial or non-commercial enzyme-linked immunosorbent assays (ELISAs) for bovine herpesvirus 1 (BHV1)-specific antibody detection in serum were compared using 41 bovine sera of well defined origin. All ELISAs were able to detect correctly most of the antibody negative sera (specificity greater-than-or-equal-to 92%). The ability, however, to detect specific antibodies varied considerably between ELISAs. Sensitivity, estimated by testing 18 positive sera, ranged between 50% and 100%. Sera with titers of at least 64, as measured by the 24 h virus neutralisation test. were identified as being positive by all ELISAs. Most assays were unable to detect specific IgM antibodies present in sera collected 9 days after experimental infection. Only one assay, an indirect ELISA using undiluted test serum, showed a sensitivity of 100%. This ELISA was found to be 8 times more sensitive than the 24 h neutralisation test and had the unique property of showing a weak consistently positive response with some sera collected from breeding bulls. The findings of this study indicate the need for international standardisation of tests to detect BHV1-specific antibodies in cattle.