MOLECULAR-CLONING OF CDNA-ENCODING THE 110-KDA AND 21-KDA REGULATORY SUBUNITS OF SMOOTH-MUSCLE PROTEIN PHOSPHATASE-1M

被引：124
作者：
CHEN, YH
CHEN, MX
ALESSI, DR
CAMPBELL, DG
SHANAHAN, C
COHEN, P
COHEN, PTW
机构：
[1] UNIV DUNDEE,DEPT BIOCHEM,MRC,PROT PHOSPHORYLAT UNIT,DUNDEE DD1 4HN,SCOTLAND
[2] ADDENBROOKES HOSP,DEPT MED,CAMBRIDGE,ENGLAND
来源：
FEBS LETTERS | 1994年 / 356卷 / 01期
关键词：
SMOOTH MUSCLE; PROTEIN PHOSPHATASE; CDNA SEQUENCE; MYOSIN; ANKYRIN REPEAT; LEUCINE ZIPPER;
D O I：
10.1016/0014-5793(94)01231-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The structures of the M(110) and M(21) regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M(110) subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M(21) subunit from chicken gizzard contains a leucine zipper motif. The M(110) subunit is expressed in two different forms which differ in their C-terminal sequences. One of these is highly homologous to the whole of the M(21) subunit.
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页码：51 / 55
页数：5
相关论文
共 23 条
  • [1] COMPLETE PRIMARY STRUCTURE OF PROTEIN PHOSPHATASE INHIBITOR-1 FROM RABBIT SKELETAL-MUSCLE
    AITKEN, A
    BILHAM, T
    COHEN, P
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1982, 126 (02): : 235 - 246
  • [2] THE CONTROL OF PROTEIN PHOSPHATASE-1 BY TARGETING SUBUNITS - THE MAJOR MYOSIN PHOSPHATASE IN AVIAN SMOOTH-MUSCLE IS A NOVEL FORM OF PROTEIN PHOSPHATASE-1
    ALESSI, D
    MACDOUGALL, LK
    SOLA, MM
    IKEBE, M
    COHEN, P
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 210 (03): : 1023 - 1035
  • [3] INHIBITOR-2 FUNCTIONS LIKE A CHAPERONE TO FOLD 3 EXPRESSED ISOFORMS OF MAMMALIAN PROTEIN PHOSPHATASE-1 INTO A CONFORMATION WITH THE SPECIFICITY AND REGULATORY PROPERTIES OF THE NATIVE ENZYME
    ALESSI, DR
    STREET, AJ
    COHEN, P
    COHEN, PTW
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 213 (03): : 1055 - 1066
  • [4] 3 GENES FOR PROTEIN PHOSPHATASE-1 MAP TO DIFFERENT HUMAN-CHROMOSOMES - SEQUENCE, EXPRESSION AND GENE LOCALIZATION OF PROTEIN SERINE/THREONINE PHOSPHATASE-1-BETA (PPP1CB)
    BARKER, HM
    BREWIS, ND
    STREET, AJ
    SPURR, NK
    COHEN, PTW
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1994, 1220 (02): : 212 - 218
  • [5] ISOLATION AND SEQUENCE-ANALYSIS OF A CDNA CLONE ENCODING A TYPE-1 PROTEIN PHOSPHATASE CATALYTIC SUBUNIT - HOMOLOGY WITH PROTEIN PHOSPHATASE-2A
    BERNDT, N
    CAMPBELL, DG
    CAUDWELL, FB
    COHEN, P
    SILVA, EFD
    SILVA, OBD
    COHEN, PTW
    [J]. FEBS LETTERS, 1987, 223 (02) : 340 - 346
  • [6] SEQUENCE OF THE HUMAN GLYCOGEN-ASSOCIATED REGULATORY SUBUNIT OF TYPE-1 PROTEIN PHOSPHATASE AND ANALYSIS OF ITS CODING REGION AND MESSENGER-RNA LEVEL IN MUSCLE FROM PATIENTS WITH NIDDM
    CHEN, YH
    HANSEN, L
    CHEN, MX
    BJORBAEK, C
    VESTERGAARD, H
    HANSEN, T
    COHEN, PTW
    PEDERSEN, O
    [J]. DIABETES, 1994, 43 (10) : 1234 - 1241
  • [7] THE STRUCTURE AND REGULATION OF PROTEIN PHOSPHATASES
    COHEN, P
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 : 453 - 508
  • [8] COHEN P, 1989, J BIOL CHEM, V264, P21435
  • [9] A MYOFIBRILLAR PROTEIN PHOSPHATASE FROM RABBIT SKELETAL-MUSCLE CONTAINS THE BETA-ISOFORM OF PROTEIN PHOSPHATASE-1 COMPLEXED TO A REGULATORY SUBUNIT WHICH GREATLY ENHANCES THE DEPHOSPHORYLATION OF MYOSIN
    DENT, P
    MACDOUGALL, LK
    MACKINTOSH, C
    CAMPBELL, DG
    COHEN, P
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 210 (03): : 1037 - 1044
  • [10] FEINBERG AP, 1984, ANAL BIOCHEM, V137, P266
123