UPTAKE AND TRANSPORT OF GLUCAGON BY RAT-LIVER - EVIDENCE FOR TRANSCYTOTIC PATHWAY

被引:0
作者
IWANIJ, V [1 ]
STUKENBROK, H [1 ]
机构
[1] YALE UNIV,SCH MED,CELL BIOL SECT,NEW HAVEN,CT
关键词
AUTORADIOGRAPHY; BILIARY SECRETION; ENDOCYTOSIS; GLUCAGON; HEPATOCYTES; TRANSCYTOSIS;
D O I
10.1007/BF01280372
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The binding of I-125-glucagon to the cell surface and the pathway of intracellular transport of this hormone by rat hepatocytes in vivo were studied by light and EM autoradiography. Radiolabeled glucagon injected into the blood stream was taken up predominantly by the hepatocytes. Negligible radioactivity was found to be associated with other cell types such as endothelial or Kupffer cells. Our results indicate that at early time points after injection glucagon has been preferentially interacting with the sinusoidal domain of the hepatocytes and found to be associated with coated pits and uncoated vesicles corresponding to endosomes. At 15-20 min time intervals glucagon grains were found within hepatocyte interior. Later, at 30 min after injection glucagon grains accumulate in the Golgi-lysosomal region of hepatocyte often in close proximity to the opening of the bile canaliculi. Accordingly a portion of internalized I-125-glucagon was found to be released into the bile thereby indicating that a transcytotic pathway may be involved in this peptides clearance process.
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页码:202 / 212
页数:11
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