ALTERATIONS OF ANGIOTENSIN-II RECEPTOR CONTENTS IN HYPERTROPHIED HEARTS

被引:39
作者
FUJII, N
TANAKA, M
OHNISHI, J
YUKAWA, K
TAKIMOTO, E
SHIMADA, S
NARUSE, M
SUGIYAMA, F
YAGAMI, K
MURAKAMI, K
MIYAZAKI, H
机构
[1] UNIV TSUKUBA,CTR GENE EXPT,INST APPL BIOCHEM,TSUKUBA,IBARAKI 305,JAPAN
[2] UNIV TSUKUBA,LAB ANIM RES CTR,TSUKUBA,IBARAKI 305,JAPAN
[3] TOKYO WOMENS MED COLL,INST CLIN ENDOCRINOL,DEPT MED,SHINJUKU KU,TOKYO 162,JAPAN
关键词
D O I
10.1006/bbrc.1995.1973
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tsukuba hypertensive mice, which carry the human genes for renin and angiotensinogen, show cardiac hypertrophy as well as hypertension due to activation of the renin-angiotensin system (RAS). Here, we compared the cardiac angiotensin II (Ang II) receptor contents in these and normotensive control mice by means of Ligand binding studies and competitive reverse transcription-polymerase chain reaction analyses. The content of the Ang II receptor type 1 (AT(1)) was significantly higher at both the protein (2.5-fold; p < 0.01) and mRNA (1.4-fold; p < 0.05) levels in the hypertensive mice than that in control mice. Almost identical levels of the Ang II receptor type 2 (AT(2)) expression were identified at the mRNA levels in the two types of mice, although the levels were less than 20% of those of AT(1) mRNA in control mice. These results suggest that AT(1) in the heart is upregulated in response to Ang II-induced hypertrophic change and that, in particular, the upregulation of AT(1) in particular contributes to the development and/or maintenance of cardiac hypertrophy in conjunction with the increase in Ang II production, because ATI is responsible for cardiac hypertrophy related to the RAS. (C) 1995 Academic Press, Inc.
引用
收藏
页码:326 / 333
页数:8
相关论文
共 25 条
  • [1] BADER M, 1994, HYPERTENS RES, V17, P1
  • [2] THE ANGIOTENSIN-AT2 RECEPTOR STIMULATES PROTEIN TYROSINE PHOSPHATASE-ACTIVITY AND MEDIATES INHIBITION OF PARTICULATE GUANYLATE-CYCLASE
    BOTTARI, SP
    KING, IN
    REICHLIN, S
    DAHLSTROEM, I
    LYDON, N
    DEGASPARO, M
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 183 (01) : 206 - 211
  • [3] CAMBELLBOSWELL M, 1981, EXP MOL PATHOL, V35, P265
  • [4] CULTURE OF RENAL ARTERIOLAR SMOOTH-MUSCLE CELLS - MITOGENIC RESPONSES TO ANGIOTENSIN-II
    DUBEY, RK
    ROY, A
    OVERBECK, HW
    [J]. CIRCULATION RESEARCH, 1992, 71 (05) : 1143 - 1152
  • [5] FUKAMIZU A, 1993, J BIOL CHEM, V268, P11617
  • [6] IDENTIFICATION OF 2 SUBTYPES IN THE RAT TYPE-I ANGIOTENSIN-II RECEPTOR
    IWAI, N
    INAGAMI, T
    [J]. FEBS LETTERS, 1992, 298 (2-3) : 257 - 260
  • [7] KAMBAYASHI Y, 1993, J BIOL CHEM, V268, P24543
  • [8] ANGIOTENSIN-II TYPE-2 RECEPTOR STIMULATION OF NEURONAL K+ CURRENTS INVOLVES AN INHIBITORY GTP-BINDING PROTEIN
    KANG, J
    POSNER, P
    SUMNERS, C
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1994, 267 (05): : C1389 - C1397
  • [9] EFFECTS OF LONG-TERM ANGIOTENSIN CONVERTING ENZYME-INHIBITION ON MYOCARDIAL HYPERTROPHY IN EXPERIMENTAL AORTIC-STENOSIS IN THE RAT
    KROMER, EP
    RIEGGER, GAJ
    [J]. AMERICAN JOURNAL OF CARDIOLOGY, 1988, 62 (01) : 161 - 163
  • [10] DISTRIBUTION AND FUNCTION OF CARDIAC ANGIOTENSIN AT(1)-RECEPTOR AND AT(2)-RECEPTOR SUBTYPES IN HYPERTROPHIED RAT HEARTS
    LOPEZ, JJ
    LORELL, BH
    INGELFINGER, JR
    WEINBERG, EO
    SCHUNKERT, H
    DIAMANT, D
    TANG, SS
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1994, 267 (02): : H844 - H852