AUTOANTIGENIC EPITOPES ON DNA TOPOISOMERASE-I - CLINICAL AND IMMUNOGENETIC ASSOCIATIONS IN SYSTEMIC-SCLEROSIS

被引：39

作者：

KUWANA, M ^{[1
]}

KABURAKI, J ^{[1
]}

MIMORI, T ^{[1
]}

TOJO, T ^{[1
]}

HOMMA, M ^{[1
]}

机构：

[1] KEIO UNIV, SCH MED, DEPT INTERNAL MED, TOKYO 108, JAPAN

来源：

ARTHRITIS AND RHEUMATISM | 1993年 / 36卷 / 10期

关键词：

D O I：

10.1002/art.1780361013

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective. To elucidate the clinical and immunogenetic associations with reactivity to autoantigenic epitopes on DNA topoisomerase I (topo I) recognized by sera from patients with systemic sclerosis (SSc). Methods. Autoantigenic epitopes on topo I were identified by screening an epitope library constructed from topo I complementary DNA restriction fragments using autoimmune anti-topo I-positive sera as a probe. Epitope reactivities of sera from 43 anti-topo I-positive SSc patients were surveyed by immunoblotting, and associations with clinical symptoms and HLA-DR types were examined. Results. Four different epitope regions were identified on the topo I molecule. Immunoreactivity to the region encompassing amino acid residues 658-700, termed ER4, was found to be associated with diffuse cutaneous SSc, progressive pulmonary interstitial fibrosis, and poor prognosis for 15-year survival. SSc patients with ER4 reactivity frequently displayed the DR2/DRw52 phenotype. Conclusion. Molecular analysis of precise antigenic epitopes on topo I is helpful in classifying clinical subsets of SSc.

引用

页码：1406 / 1413

页数：8

共 35 条

[1]

AIZAWA M, 1986, HLA ASIA OCEANIA 198

[2]

[Anonymous], 1979, AM REV RESPIR DIS, V119, P831

[3] PRELIMINARY CRITERIA FOR THE CLASSIFICATION OF SYSTEMIC-SCLEROSIS (SCLERODERMA) [J].

不详 .

ARTHRITIS AND RHEUMATISM, 1980, 23 (05) :581-590

[4]

BALDWIN ED, 1948, MEDICINE, V27, P243

[5] IMMUNOGENETIC PREDICTION OF PULMONARY FIBROSIS IN SYSTEMIC-SCLEROSIS [J].

BRIGGS, DC ;

VAUGHAN, RW ;

WELSH, KI ;

MYERS, A ;

DUBOIS, RM ;

BLACK, CM .

LANCET, 1991, 338 (8768) :661-662

[6] SEROLOGICAL MARKERS IN PROGRESSIVE SYSTEMIC-SCLEROSIS - CLINICAL CORRELATIONS [J].

CATOGGIO, LJ ;

BERNSTEIN, RM ;

BLACK, CM ;

HUGHES, GRV ;

MADDISON, PJ .

ANNALS OF THE RHEUMATIC DISEASES, 1983, 42 (01) :23-27

[7] CDNA CLONING OF HUMAN DNA TOPOISOMERASE-I - CATALYTIC ACTIVITY OF A 67.7-KDA CARBOXYL-TERMINAL FRAGMENT [J].

DARPA, P ;

MACHLIN, PS ;

RATRIE, H ;

ROTHFIELD, NF ;

CLEVELAND, DW ;

EARNSHAW, WC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (08) :2543-2547

[8] USE OF MOLECULAR-CLONING METHODS TO MAP THE DISTRIBUTION OF EPITOPES ON TOPOISOMERASE-I (SCL-70) RECOGNIZED BY SERA OF SCLERODERMA PATIENTS [J].

DARPA, P ;

WHITECOOPER, H ;

CLEVELAND, DW ;

ROTHFIELD, NF ;

EARNSHAW, WC .

ARTHRITIS AND RHEUMATISM, 1990, 33 (10) :1501-1511

[9]

GULDNER HH, 1988, J IMMUNOL, V141, P469

[10] THE LUPUS AUTOANTIGENS AND THE PATHOGENESIS OF SYSTEMIC LUPUS-ERYTHEMATOSUS [J].

HARDIN, JA .

ARTHRITIS AND RHEUMATISM, 1986, 29 (04) :457-460

← 1 2 3 4 →