CATABOLITE INACTIVATION OF THE YEAST MALTOSE TRANSPORTER IS DUE TO PROTEOLYSIS

被引:47
作者
LUCERO, P
HERWEIJER, M
LAGUNAS, R
机构
[1] CSIC, INST INVEST BIOMED, ARTUTO DUPERIER 4, E-28029 MADRID, SPAIN
[2] GIST BROCADES, 2600 MA DELFT, NETHERLANDS
关键词
CATABOLITE INACTIVATION; MALTOSE TRANSPORT; TRANSPORT INACTIVATION; TRANSPORT TURNOVER; SACCHAROMYCES-CEREVISIAE;
D O I
10.1016/0014-5793(93)80397-D
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The maltose transport capacity of fermenting Saccharomyces cerevisiae rapidly decreases when protein synthesis is impaired. Using polyclonal antibodies against a recombinant maltose transporter-protein we measured the cellular content of the transporter along this inactivation process. Loss of transport capacity was paralleled by a decrease of cross-reacting material which suggests degradation of the transporter. We also show that in ammonium-starved cells the half-life of the maltose transporter is 1.3 h during catabolism of glucose and > 15 h during catabolism of ethanol.
引用
收藏
页码:165 / 168
页数:4
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