Plantlet regeneration from mature embryos of peanut (Arachis hypogaea L)

Mature embryos, excised from the soaked peanut seeds, when cultured on MS medium supplemented with BA and 2,4-D each @ 0.5 mg/l induced, callus. Regeneration occurred when this callus was subcultured on to MS containing BA and kin each @ 1.0 mg/l. Similarly, development of callus followed by regeneration was noticed in mature embryos in MS medium containing 0.5 mg/l kin and 1.5 mg/l NAA. Direct regeneration was also observed on MS containing 1.0 mg/l each of BA and kin. All the regenerated shoots when transferred to the rooting medium (1/2 MS) supplemented with 0.1 mg/l NAA showed development of root.