INHIBITION OF COLON-CANCER CELL-PROLIFERATION BY ANTISENSE OLIGONUCLEOTIDES TARGETING THE MESSENGER-RNA OF THE KI-RAS GENE

被引:16
作者
SAKAKURA, C [1 ]
HAGIWARA, A [1 ]
TSUJIMOTO, H [1 ]
OZAKI, K [1 ]
SAKAKIBARA, T [1 ]
OYAMA, T [1 ]
OGAKI, M [1 ]
IMANISHI, T [1 ]
YAMAZAKI, J [1 ]
TAKAHASHI, T [1 ]
机构
[1] KYOTO PREFECTURAL UNIV MED,DEPT SURG 1,KAMIKYO KU,KYOTO 602,JAPAN
关键词
ANTISENSE PHOSPHOROTHIOATE OLIGONUCLEOTIDES; ANTISENSE-MEDIATED CHANGE; COLON CANCER; GENE REGULATION KI-RAS;
D O I
10.1097/00001813-199508000-00008
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Point mutations that activate the Ki-ras proto-oncogene are present in approximately 50% of human colorectal tumors and the activated Ki-ras gene is considered to play an important role in colorectal cancer cell proliferation, Five different colon cancer cell lines and two kinds of control cell lines were treated with antisense oligonucleotides complementary to the messenger RNA of Ki-ras, Treatment with antisense oligonucleotides at concentrations between 10 and 40 mu M significantly and dose-dependently inhibited cell growth, colony formation and Ki-ras protein production of the colon cancer cells with activated Ki-ras, but did not affect the normal cells and colon cancer cells without Ki-ras mutation, These results show that use of synthetic oligonucleotides is an effective way of producing antisense-mediated changes in the behavior of human colon cancer cells with an activated Ki-ras gene.
引用
收藏
页码:553 / 561
页数:9
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