REVERSIBLE DISSOCIATION AND UNFOLDING OF THE DIMERIC PROTEIN THYMIDYLATE SYNTHASE

被引：20

作者：

PERRY, KM ^{[1
]}

POOKANJANATAVIP, M ^{[1
]}

ZHAO, J ^{[1
]}

SANTI, DV ^{[1
]}

STROUD, RM ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,DEPT PHARMACEUT CHEM,SAN FRANCISCO,CA 94143

来源：

PROTEIN SCIENCE | 1992年 / 1卷 / 06期

关键词：

DIMERIZATION; FOLDING; OLIGOMERIZATION; THYMIDYLATE SYNTHASE;

D O I：

10.1002/pro.5560010611

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Conditions for in vitro unfolding and refolding of dimeric thymidylate synthase from Lactobacillus casei were found. Ultraviolet difference and circular dichroism spectra showed that the enzyme was completely unfolded at concentrations of urea over 5.5 M. As measured by restoration of enzyme activity, refolding was accomplished when 0.5 M potassium chloride was included in the refolding mixture. Recombination of subunits from catalytically inactive mutant homodimers to form an active hybrid dimer was achieved under these unfolding-refolding conditions, demonstrating a monomer to dimer association step.

引用

页码：796 / 800

页数：5

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