THE DIRECT MEASUREMENT OF PROTEIN-KINASE-C (PKC) ACTIVITY IN ISOLATED MEMBRANES USING A SELECTIVE PEPTIDE SUBSTRATE

被引：128
作者：
CHAKRAVARTHY, BR
BUSSEY, A
WHITFIELD, JF
SIKORSKA, M
WILLIAMS, RE
DURKIN, JP
机构：
[1] NATL RES COUNCIL CANADA,INST BIOL SCI,CELL SIGNAL GRP,CELL SYST SECT,BLDG M54,OTTAWA K1A 0R6,ONTARIO,CANADA
[2] NATL RES COUNCIL CANADA,INST BIOL SCI,PROT ENGN & DESIGN SECT,OTTAWA K1A 0R6,ONTARIO,CANADA
来源：
ANALYTICAL BIOCHEMISTRY | 1991年 / 196卷 / 01期
关键词：
D O I：
10.1016/0003-2697(91)90130-L
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A protein kinase C (PKC)-selective peptide substrate was used to develop a method for measuring PKC activity directly and quantitatively in isolated cell membranes without prior detergent extraction and reconstitution of the enzyme with phosphatidylserine and TPA in the presence of excess Ca2+. This simple and rapid method can reliably measure changes in membrane-associated PKC activity induced by various bioactive compounds such as hormones and growth factors. Also, this method, which measures PKC activity in its native membrane-associated state, has the advantage of being able to distinguish between active and inactive PKC associated with cell membranes. © 1991.
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收藏
页码：144 / 150
页数：7
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