FUSARIUM-SOLANI-PISI RECOMBINANT CUTINASE PARTITIONING IN PEG POTASSIUM PHOSPHATE AQUEOUS 2-PHASE SYSTEMS

被引：10
|
作者：
SEBASTIAO, MJ
CABRAL, JMS
AIRESBARROS, MR
机构：
[1] Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, Lisboa
来源：
BIOTECHNOLOGY TECHNIQUES | 1993年 / 7卷 / 09期
关键词：
D O I：
10.1007/BF00151860
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An aqueous two-phase system of polyethylene glycol (PEG) and potassium phosphate was developed for extraction of a cutinase from cell debris of a recombinant Escherichia coli strain. Basic studies to identify the primary factors which affect cutinase partition, namely the influence of polymer molecular weight, polymer concentration and pH were carried out using a purified preparation of the cutinase. The enzyme partition coefficient was enhanced with decreasing PEG molecular weight, increasing tie-line length and pH.
引用
收藏
页码：631 / 634
页数：4
相关论文
共 50 条
  • [41] PREDICTING PARTITION-COEFFICIENTS IN POLYETHYLENE-GLYCOL POTASSIUM PHOSPHATE AQUEOUS 2-PHASE SYSTEMS
    EITEMAN, MA
    GAINER, JL
    JOURNAL OF CHROMATOGRAPHY, 1991, 586 (02): : 341 - 346
  • [42] FUNDAMENTAL-STUDIES OF BIOMOLECULE PARTITIONING IN AQUEOUS 2-PHASE SYSTEMS
    DIAMOND, AD
    HSU, JT
    BIOTECHNOLOGY AND BIOENGINEERING, 1989, 34 (07) : 1000 - 1014
  • [43] A LATTICE MODEL FOR AFFINITY PARTITIONING IN 2-PHASE AQUEOUS POLYMER SYSTEMS
    BASKIR, JN
    HATTON, TA
    SUTER, UW
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1988, 195 : 150 - IEC
  • [44] MOLECULAR-MASS OF PROTEINS AND THEIR PARTITIONING IN AQUEOUS 2-PHASE SYSTEMS
    ANDREASEN, PA
    JOURNAL OF CHROMATOGRAPHY A, 1994, 664 (01) : 99 - 103
  • [45] THE EFFECT OF THE PH DIFFERENCE BETWEEN PHASES ON PARTITIONING IN POLY(ETHYLENE GLYCOL)-PHOSPHATE AQUEOUS 2-PHASE SYSTEMS
    EITEMAN, MA
    GAINER, JL
    CHEMICAL ENGINEERING COMMUNICATIONS, 1991, 105 : 171 - 183
  • [46] Partitioning of α-lactalbumin and β-lactoglobulin in aqueous two-phase systems of polyvinylpyrrolidone and potassium phosphate
    Mokhtarani, Babak
    Mortaheb, Hamid Reza
    Mafi, Morteza
    Amini, Mohammad Hassan
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2011, 879 (11-12): : 721 - 726
  • [47] LIQUID-LIQUID-EXTRACTION OF A RECOMBINANT PROTEIN, CYTOCHROME B(5) WITH AQUEOUS 2-PHASE SYSTEMS OF POLYETHYLENE-GLYCOL AND POTASSIUM PHOSPHATE SALTS
    SARMENTO, MJ
    PIRES, MJ
    CABRAL, JMS
    AIRESBARROS, MR
    JOURNAL OF CHROMATOGRAPHY A, 1994, 668 (01) : 117 - 120
  • [48] AQUEOUS 2-PHASE SYSTEMS OF POLY(ETHYLENE GLYCOL) AND DI-POTASSIUM HYDROGEN PHOSPHATE WITH AND WITHOUT PARTITIONING BIOMOLECULES - EXPERIMENTAL RESULTS AND MODELING OF THERMODYNAMIC PROPERTIES
    GROSSMANN, C
    TINTINGER, R
    ZHU, J
    MAURER, G
    BERICHTE DER BUNSEN-GESELLSCHAFT-PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 1995, 99 (05): : 700 - 712
  • [49] PREDICTING PARTITIONING IN AQUEOUS 2-PHASE SYSTEMS AND THE EFFECTS OF TEMPERATURE-CHANGES
    COHEN, LM
    GAINER, JL
    SEPARATION SCIENCE AND TECHNOLOGY, 1994, 29 (15) : 1925 - 1942
  • [50] APPLICATION OF INTEGRAL-EQUATION THEORY TO AQUEOUS 2-PHASE PARTITIONING SYSTEMS
    HAYNES, CA
    BENITEZ, FJ
    BLANCH, HW
    PRAUSNITZ, JM
    AICHE JOURNAL, 1993, 39 (09) : 1539 - 1557
12345