FUSARIUM-SOLANI-PISI RECOMBINANT CUTINASE PARTITIONING IN PEG POTASSIUM PHOSPHATE AQUEOUS 2-PHASE SYSTEMS

被引：10
|
作者：
SEBASTIAO, MJ
CABRAL, JMS
AIRESBARROS, MR
机构：
[1] Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, Lisboa
来源：
BIOTECHNOLOGY TECHNIQUES | 1993年 / 7卷 / 09期
关键词：
D O I：
10.1007/BF00151860
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An aqueous two-phase system of polyethylene glycol (PEG) and potassium phosphate was developed for extraction of a cutinase from cell debris of a recombinant Escherichia coli strain. Basic studies to identify the primary factors which affect cutinase partition, namely the influence of polymer molecular weight, polymer concentration and pH were carried out using a purified preparation of the cutinase. The enzyme partition coefficient was enhanced with decreasing PEG molecular weight, increasing tie-line length and pH.
引用
收藏
页码：631 / 634
页数：4
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