FUSARIUM-SOLANI-PISI RECOMBINANT CUTINASE PARTITIONING IN PEG POTASSIUM PHOSPHATE AQUEOUS 2-PHASE SYSTEMS

被引：10

作者：

SEBASTIAO, MJ

CABRAL, JMS

AIRESBARROS, MR

机构：

[1] Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, Lisboa

来源：

BIOTECHNOLOGY TECHNIQUES | 1993年 / 7卷 / 09期

关键词：

D O I：

10.1007/BF00151860

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An aqueous two-phase system of polyethylene glycol (PEG) and potassium phosphate was developed for extraction of a cutinase from cell debris of a recombinant Escherichia coli strain. Basic studies to identify the primary factors which affect cutinase partition, namely the influence of polymer molecular weight, polymer concentration and pH were carried out using a purified preparation of the cutinase. The enzyme partition coefficient was enhanced with decreasing PEG molecular weight, increasing tie-line length and pH.

引用

页码：631 / 634

页数：4

共 10 条

[1]

Albertsson P. A., 1990, SEPARATION PROCESSES, P287

[2] A CORRELATION BETWEEN CLOUDING AND AQUEOUS BIPHASE FORMATION IN POLYETHYLENE OXIDE INORGANIC SALT SYSTEMS [J].