PURIFICATION AND CHARACTERIZATION OF A 26-KDA COMPETITIVE INHIBITOR OF INTERLEUKIN-1

被引：76

作者：

MAZZEI, GJ ^{[1
]}

SECKINGER, PL ^{[1
]}

DAYER, JM ^{[1
]}

SHAW, AR ^{[1
]}

机构：

[1] HOP CANTONAL GENEVA,DEPT IMMUNOL & ALLERGY,CH-1211 GENEVA 4,SWITZERLAND

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 1990年 / 20卷 / 03期

关键词：

D O I：

10.1002/eji.1830200332

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The urine of patients with fever above 39 °C contains an inhibitor of interleukin 1. Herein we describe the purification of the interleukin 1 inhibitor by ion‐exchange chromatography, hydrophobic chromatography, gel filtration and negative immunosorption. The purified protein has a molecular weight of 26000; its size is reduced to 24000 upon treatment with endoglycosidase F. The IL 1 inhibitor is a competitive inhibitor and acts by binding to the IL 1 receptor. The inhibitor recognizes the murine and human IL 1 receptor with similar affinity. The purified IL 1 inhibitor has a specific activity of 3 × 107 to 4.5 × 107 units/mg as tested on the human astrocytoma (U‐373) cell proliferation bioassay and murine EL4.6.1 cell IL 1 receptor‐binding assay. Copyright © 1990 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim

引用

页码：683 / 689

页数：7

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