MITOGENIC ACTIVITY OF PROCATHEPSIN-D PURIFIED FROM CONDITIONED MEDIUM OF BREAST-CANCER CELLS BY AFFINITY-CHROMATOGRAPHY ON PEPSTATINYL AGAROSE

被引：24

作者：

STEWART, AJ ^{[1
]}

PIGGOTT, NH ^{[1
]}

MAY, FEB ^{[1
]}

WESTLEY, BR ^{[1
]}

机构：

[1] ROYAL VICTORIA INFIRM,DEPT PATHOL,NEWCASTLE TYNE NE1 4LP,TYNE & WEAR,ENGLAND

来源：

INTERNATIONAL JOURNAL OF CANCER | 1994年 / 57卷 / 05期

基金：

英国惠康基金;

关键词：

D O I：

10.1002/ijc.2910570518

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A simple procedure for the affinity purification of procathepsin D from tissue culture medium conditioned by breast-cancer cells is described. This procedure yielded 2 mu g of procathepsin D/100 ml medium. The procathepsin D was approximately 95% pure as judged by silver staining of polyacrylamide gels, the major contaminant being mature cathepsin D. The ability of procapthesin D to stimulate the proliferation of oestrogen-responsive MCF-7 breast cancer cells was determined. The purified procapthesin D had no mitogenic effect alone or in combination with oestradiol or other growth factors. These data suggest that procapthesin D does not act as an oestrogen-regulated autocrine growth factor for malignant breast epithelial calls. (C) 1994 Wiley-Liss, Inc.

引用

页码：715 / 718

页数：4

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