CLONING OF MESSENGER-RNA FOR SOYBEAN SEED PROTEINS

被引：0
|
作者：
EPISHIN, SM [1 ]
KISELEV, SL [1 ]
ZAKHAROVA, ES [1 ]
BOLOTIN, AP [1 ]
RYSKOV, AP [1 ]
VINETSKI, YP [1 ]
机构：
[1] ACAD SCI USSR, INST MOLEC BIOL, MOSCOW V-71, USSR
来源：
GENETIKA | 1985年 / 21卷 / 02期
关键词：
D O I：
暂无
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The set of polypeptides was studied after translation of soybean seed mRNA in rabbit reticulocyte system. Storage proteins of soybean-glycinin and conglycinin are synthesized as a high-MW precursors. The precursors of mature subunits of glycinin had a MW approaching the sum of MW of basic and acidic subunits; the precursors of conglycinin were 8000 daltons longer than mature ones. The mRNA sequences were cloned after reverse transcription into pBR322. The c[complementary]DNA clones containing the structural sequences of 2 subunits of conglycinin. The glycinin precursor and 8800 dalton polypeptide were identified by hybrid-selection procedure. A portion of the gene of one conglycinin subunit was sequenced.
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页码：210 / 215
页数：6
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