CLONING OF MESSENGER-RNA FOR SOYBEAN SEED PROTEINS

被引：0

作者：

EPISHIN, SM ^{[1
]}

KISELEV, SL ^{[1
]}

ZAKHAROVA, ES ^{[1
]}

BOLOTIN, AP ^{[1
]}

RYSKOV, AP ^{[1
]}

VINETSKI, YP ^{[1
]}

机构：

[1] ACAD SCI USSR, INST MOLEC BIOL, MOSCOW V-71, USSR

来源：

GENETIKA | 1985年 / 21卷 / 02期

关键词：

D O I：

暂无

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The set of polypeptides was studied after translation of soybean seed mRNA in rabbit reticulocyte system. Storage proteins of soybean-glycinin and conglycinin are synthesized as a high-MW precursors. The precursors of mature subunits of glycinin had a MW approaching the sum of MW of basic and acidic subunits; the precursors of conglycinin were 8000 daltons longer than mature ones. The mRNA sequences were cloned after reverse transcription into pBR322. The c[complementary]DNA clones containing the structural sequences of 2 subunits of conglycinin. The glycinin precursor and 8800 dalton polypeptide were identified by hybrid-selection procedure. A portion of the gene of one conglycinin subunit was sequenced.

引用

页码：210 / 215

页数：6

共 14 条

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