TGF-BETA-1 AND 25-HYDROXYCHOLESTEROL STIMULATE OSTEOBLAST-LIKE VASCULAR CELLS TO CALCIFY

被引:413
作者
WATSON, KE [1 ]
BOSTROM, K [1 ]
RAVINDRANATH, R [1 ]
LAM, T [1 ]
NORTON, B [1 ]
DEMER, LL [1 ]
机构
[1] UNIV CALIF LOS ANGELES,SCH MED,CTR HLTH SCI 47123,DEPT MED,DIV CARDIOL,LOS ANGELES,CA 90024
关键词
ATHEROSCLEROSIS; CALCIFICATION; OXYSTEROL; PERICYTES; GANGLIOSIDES;
D O I
10.1172/JCI117205
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Previous studies in our laboratory demonstrated messenger RNA for bone morphogenetic protein-2a in human calcified plaque, suggesting that arterial calcification is a regulated process, similar to osteogenesis. To further test this hypothesis, we have isolated and cloned a subpopulation of cells from bovine aortic media that show osteoblastic potential. These novel cells are primarily distinguished from smooth muscle cells by expression of a surface marker preliminarily identified as a modified form of the ganglioside sialyl-lactosylceramide (GM3). Osteoblastic potential was indicated by high levels of alkaline phosphatase and collagen I, expression of osteopontin and osteonectin (SPARC), and production of bone-specific osteocalcin and hydroxyapatite. Cultures of these cells were stimulated to form increased numbers of calcium-mineral-producing nodules by the oxysterol 25-hydroxycholesterol as well as by transforming growth factor-beta 1, both known to be present in atherosclerotic lesions. The stimulation of calcifying vascular cells in the artery wall by these two factors suggests a possible mechanism for the colocalization of calcification with atherosclerosis in vivo.
引用
收藏
页码:2106 / 2113
页数:8
相关论文
共 47 条
[1]   QUANTIFICATION OF CORONARY-ARTERY CALCIUM USING ULTRAFAST COMPUTED-TOMOGRAPHY [J].
AGATSTON, AS ;
JANOWITZ, WR ;
HILDNER, FJ ;
ZUSMER, NR ;
VIAMONTE, M ;
DETRANO, R .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 1990, 15 (04) :827-832
[2]  
ANDERSON HC, 1983, ARCH PATHOL LAB MED, V107, P341
[3]   GROWTH ON TYPE-I COLLAGEN PROMOTES EXPRESSION OF THE OSTEOBLASTIC PHENOTYPE IN HUMAN OSTEOSARCOMA MG-63 CELLS [J].
ANDRIANARIVO, AG ;
ROBINSON, JA ;
MANN, KG ;
TRACY, RP .
JOURNAL OF CELLULAR PHYSIOLOGY, 1992, 153 (02) :256-265
[4]  
Baroldi G, 1988, Am J Cardiovasc Pathol, V2, P159
[5]  
BEADENKOPF WG, 1964, AMER J ROENTGENOL RA, V92, P865
[6]   BONE MORPHOGENETIC PROTEIN EXPRESSION IN HUMAN ATHEROSCLEROTIC LESIONS [J].
BOSTROM, K ;
WATSON, KE ;
HORN, S ;
WORTHAM, C ;
HERMAN, IM ;
DEMER, LL .
JOURNAL OF CLINICAL INVESTIGATION, 1993, 91 (04) :1800-1809
[7]  
BRIGHTON CT, 1992, CLIN ORTHOP RELAT R, V275, P287
[8]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[9]  
DAMORE PA, 1990, CELL CULTURE TECHNIQ, P299
[10]  
DIAZFLORES L, 1990, HISTOL HISTOPATHOL, V5, P145