ANALYSIS OF LARGE DNA FROM SOYBEAN (GLYCINE-MAX L MERR) BY PULSED-FIELD GEL-ELECTROPHORESIS

被引：0

作者：

HONEYCUTT, RJ ^{[1
]}

SOBRAL, BWS ^{[1
]}

MCCLELLAND, M ^{[1
]}

ATHERLY, AG ^{[1
]}

机构：

[1] IOWA STATE UNIV SCI & TECHNOL, DEPT GENET, AMES, IA 50011 USA

来源：

PLANT JOURNAL | 1992年 / 2卷 / 01期

关键词：

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The technique of pulsed-field gel electrophoresis (PFE) has been used to study chromosomal regions and entire genomes of several organisms. Techniques are presented for the isolation of high molecular weight DNA from embedded soybean protoplasts and the conditions for separating large DNA fragments using PFE. Digestion was detected by Southern hybridization using single copy nodulin clones. These data are being used to generate a physical map of the nodulin region(s) of the soybean genome.

引用

页码：133 / 135

页数：3

共 20 条

[1] NUCLEAR-DNA AMOUNTS IN ANGIOSPERMS [J].

BENNETT, MD ;

SMITH, JB .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1976, 274 (933) :227-274

[2] NUCLEAR-DNA AMOUNTS IN ANGIOSPERMS [J].

BENNETT, MD ;

SMITH, JB ;

HESLOPHARRISON, JS .

PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1982, 216 (1203) :179-199

[3] THE ISOLATION OF HIGH-MOLECULAR-WEIGHT DNA FROM WHEAT, BARLEY AND RYE FOR ANALYSIS BY PULSE-FIELD GEL-ELECTROPHORESIS [J].

CHEUNG, WY ;

GALE, MD .

PLANT MOLECULAR BIOLOGY, 1990, 14 (05) :881-888

[4] THE CHLOROPLAST GENOME EXISTS IN MULTIMERIC FORMS [J].

DENG, XW ;

WING, RA ;

GRUISSEM, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (11) :4156-4160

[5] PREPARATION OF PLANT DNA FOR SEPARATION BY PULSED FIELD GEL-ELECTROPHORESIS [J].

DEVOS, KM ;

VERCRUYSSEDEWITTE, D .

ELECTROPHORESIS, 1989, 10 (04) :267-268

[6] NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS [J].

GAMBORG, OL ;

MILLER, RA ;

OJIMA, K .

EXPERIMENTAL CELL RESEARCH, 1968, 50 (01) :151-+

[7] PULSED FIELD GEL-ELECTROPHORESIS AND PHYSICAL MAPPING OF LARGE DNA FRAGMENTS IN THE TM-2A REGION OF CHROMOSOME-9 IN TOMATO [J].

GANAL, MW ;

YOUNG, ND ;

TANKSLEY, SD .

MOLECULAR AND GENERAL GENETICS, 1989, 215 (03) :395-400

[8]

GANAL MW, 1989, PLANT MOL BIOL REP, V7, P17

[9] SEQUENCE ORGANIZATION OF THE SOYBEAN GENOME [J].

GURLEY, WB ;

HEPBURN, AG ;

KEY, JL .

BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 561 (01) :167-183

[10]

KEIM P, 1990, GENETICS, V126, P735

← 1 2 →