A VACUUM COLLECTION AND SEED SEPARATION TECHNIQUE FOR ENUMERATION OF SCLEROTIA OF CLAVICEPS-PURPUREA IN PERENNIAL RYEGRASS FIELDS

被引：3

作者：

ALDERMAN, SC ^{[1
]}

CHURCHILL, DB ^{[1
]}

BILSLAND, DM ^{[1
]}

机构：

[1] OREGON STATE UNIV,DEPT BIORESOURCE ENGN,CORVALLIS,OR 97331

来源：

PLANT DISEASE | 1993年 / 77卷 / 10期

关键词：

D O I：

10.1094/PD-77-1020

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A portable electric wet-dry shop vacuum run by a gas-powered generator was used to collect sclerotia of Claviceps purpurea from 1-m2 areas in a commercial field of perennial ryegrass. Sclerotia were separated from soil and straw residue using an air-screen (seed separation) machine, in which samples were partitioned based on particle width and thickness, and terminal velocity. The procedure also was used to determine the number of sclerotia in bales of annual ryegrass straw. Efficiency of recovery of sclerotia using the air-screen machine with known numbers of ergot was 98-100%. Recovery by vacuum collection in the field of a known number of sclerotia distributed over surface residue and plant crowns was 83% +/- 4% in areas without soil cracks, 73% +/- 15% in areas with deep soil cracks, and 79% +/- 13% in areas selected at random. In perennial ryegrass with 23% of heads infected (two sclerotia per infected head), 39 sclerotia per square meter of soil surface were recovered.

引用

页码：1020 / 1022

页数：3