SEX AND DEVELOPMENT IN BOVINE INVITRO FERTILIZED EMBRYOS

被引：144
作者：
AVERY, B
MADISON, V
GREVE, T
机构：
[1] Department of Reproduction, Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C
来源：
THERIOGENOLOGY | 1991年 / 35卷 / 05期
关键词：
SEXING; CATTLE; INVITRO FERTILIZATION; DIFFERENTIAL GROWTH RATE;
D O I：
10.1016/0093-691X(91)90306-X
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In vitro fertilized bovine embryos were karyotyped at Day 7 and Day 8 post insemination. The results showed that the percentage of males (sex ratio) was dependent on the developmental stage. Among expanded blastocysts (the most advanced embryos), the sex ratio was 100%, followed by 89% for expanding blastocysts, 75% for blastocysts, and 40% for young blastocysts, all analyzed at Day 7. For embryos karyotyped at Day 8, the sex ratio was 20%. The average mitotic index for all in vitro fertilized embryos was 3.5%. These results suggest that the apparent relationship between sex and developmental rate could be used as a method for noninvasive sexing of in vitro fertilized embryos.
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页码：953 / 963
页数：11
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