PROTEIN LADDER SEQUENCING

被引:259
作者
CHAIT, BT
WANG, R
BEAVIS, RC
KENT, SBH
机构
[1] SCRIPPS RES INST, LA JOLLA, CA 92037 USA
[2] ROCKEFELLER UNIV, NEW YORK, NY 10021 USA
[3] MEM UNIV NEWFOUNDLAND, DEPT PHYS, ST JOHNS A1B 3X7, NEWFOUNDLAND, CANADA
关键词
D O I
10.1126/science.8211132
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A new approach to protein sequencing is described. It consists of two steps: (i) ladder-generating chemistry, the controlled generation from a polypeptide chain by wet chemistry of a family of sequence-defining peptide fragments, each differing from the next by one amino acid; and (ii) data readout, a one-step readout of the resulting protein sequencing ladder by matrix-assisted laser-desorption mass spectrometry. Each amino acid was identified from the mass difference between successive peaks, and the position in the data set defined the sequence of the original peptide chain. This method was used to directly locate a phosphoserine residue in a phosphopeptide. The protein ladder sequencing method lends itself to very high sample throughput at very low per cycle cost.
引用
收藏
页码:89 / 92
页数:4
相关论文
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