VARIATION IN DNA FINGERPRINTS DETECTED IN PLANTS REGENERATED FROM HORSERADISH (ARMORACIA-RUSTICANA GAERTN) CALLUS

DNA fingerprints patterns were compared among regenerated plants from root- and leaf-derived calli of horseradish using digoxygenin-labeled oligonucleotide probes for Southern hybridization. Among the seven probes investigated, (GAA)(5) produced the dearest banding patterns. Two out of 14 regenerated plants from leaf-derived calli and five out of 12 plants from root-derived calli exhibited clearly different patterns from their mother plants. Except for one, these variant plants in DNA fingerprints could not be distinguished morphologically from their mother plants. Thus, DNA finterprinting is considered a possible means for detecting somaclonal variation in horseradish.