PURIFICATION OF A VASOACTIVE PEPTIDE RELATED TO LYSYL-BRADYKININ FROM TROUT PLASMA

被引：26

作者：

CONLON, JM ^{[1
]}

OLSON, KR ^{[1
]}

机构：

[1] UNIV NOTRE DAME,INDIANA UNIV SCH MED,S BEND CTR,NOTRE DAME,IN 46556

来源：

FEBS LETTERS | 1993年 / 334卷 / 01期

基金：

美国国家科学基金会;

关键词：

BRADYKININ; KALLIDIN; KININOGEN; KALLIKREIN; BLOOD PRESSURE; TROUT;

D O I：

10.1016/0014-5793(93)81684-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Incubation of plasma from the steelhead trout, Oncorhynchus mykiss with porcine pancreatic glandular kallikrein generated bradykinin-like immunoreactivity. The primary structure of the immunoreactive peptide was established as: Lys-Arg-Pro-Pro-Gly-Trp-Pro-Leu-Arg. This sequence shows two amino acid substitutions (Phe6-->Trp and Phe9-->Leu) compared with mammalian lysyl-bradykinin (kallidin). Bolus intra-arterial injection of the purified peptide produced a strong and sustained vasopressor response in the unanaesthetized trout. The data demonstrate that the kallikrein-kinin system predates the appearance of tetrapods and suggest a role for this system in cardiovascular regulation in fish.

引用

页码：75 / 78

页数：4

共 15 条

[1]

BALKS HJ, 1988, CLIN ENDOCRINOL, V28, P141

[2] BRADYKININ AND THROMBIN EFFECTS ON POLYPHOSPHOINOSITIDE HYDROLYSIS AND PROSTACYCLIN PRODUCTION IN ENDOTHELIAL-CELLS [J].