AUTOMATED INCORPORATION OF POLYETHYLENE-GLYCOL INTO SYNTHETIC OLIGONUCLEOTIDES

被引：41

作者：

JASCHKE, A ^{[1
]}

FURSTE, JP ^{[1
]}

CECH, D ^{[1
]}

ERDMANN, VA ^{[1
]}

机构：

[1] FREE UNIV BERLIN,INST BIOCHEM,W-1000 BERLIN 63,GERMANY

来源：

TETRAHEDRON LETTERS | 1993年 / 34卷 / 02期

关键词：

D O I：

10.1016/S0040-4039(00)60572-5

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

(4,4'-Dimethoxytrityl)-polyethylene glycol-(2-cyanoethyl-N,N-diisopropyl)-phosphoramidites and (4,4'-dimethoxytrityl)-polyethylene glycol derivatized glass supports were synthesized and used to introduce PEG with polymerization degrees from about 6 to more than 100 at 3'- and 5'-ends of oligonucleotides by means of automated synthesis. The electrophoretic mobility of the conjugates is altered with respect to the unmodified oligonucleotides. The hydrophobicity of the conjugates increases with increasing length of the PEG chain.

引用

页码：301 / 304

页数：4

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