HYPERPRODUCTION OF A BIFUNCTIONAL HYBRID PROTEIN, METAPYROCATECHASE-PROTEIN A, BY GENE FUSION

被引：12

作者：

KOBATAKE, E

IKARIYAMA, Y

AIZAWA, M

MIWA, K

KATO, S

机构：

[1] TOKYO INST TECHNOL,DEPT BIOENGN,MEGURO KU,OOKAYAMA 2-12-1,TOKYO 152,JAPAN

[2] SAGAMI CHEM RES CTR,GENET ENGN SECT,NISHIOHNUMA,KANAGAWA,JAPAN

来源：

JOURNAL OF BIOTECHNOLOGY | 1990年 / 16卷 / 1-2期

关键词：

Binding protein; Enzyme conjugate; Fusion protein; Recombinant DNA;

D O I：

10.1016/0168-1656(90)90067-L

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A hybrid protein between metapyrocatechase and Staphylococcal protein A was produced by recombinant DNA techniques. A plasmid carrying the fusion gene that encodes the hybrid protein was constructed and expressed in E. coli. Over 70% of soluble proteins of the cell extracts was estimated to be the hybrid protein. This fusion protein is about 65000. Both the IgG-binding activity of protein A and the metapyrocatechase activity were found in the hybrid protein. The optimum pH of metapyrocatechase in the fusion protein was at around 6.5 and Km was 1.3 × 10-5 M. A simple immuno-enzymometric assay was developed for anti-BSA antibody using the fusion protein. © 1990.

引用

页码：87 / 96

页数：10

共 18 条

[1]

Aizawa M., 1976, J SOL-PHASE BIOCHEM, V1, P319

[2] CHARACTERIZATION OF AN ARTIFICIAL BIFUNCTIONAL ENZYME, BETA-GALACTOSIDASE GALACTOKINASE, PREPARED BY GENE FUSION [J].