MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF CDNA-ENCODING HUMAN CHOLESTEROL 7-ALPHA-HYDROXYLASE

被引：101
作者：
NOSHIRO, M
OKUDA, K
机构：
[1] Department of Biochemistry, School of Dentistry, Hiroshima University, Minami-ku, Hiroshima, 734
来源：
FEBS LETTERS | 1990年 / 268卷 / 01期
关键词：
cDNA cloning; Cholesterol; 7α-hydroxylase; COS cell; Cytochrome P-450; Herne binding region; Steroid binding region;
D O I：
10.1016/0014-5793(90)80992-R
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A complete cDNA clone encoding human cholesterol 7α-hydroxylase has been isolated using a rat P-450ch7α cDNA insert [(1989) FEBS Lett. 257, 97-100] as a probe and totally sequenced. The cDNA contained 1512-base pair open reading frame encoding 504 amino acid residues (Mr, 57630), 39-base pair 5'-untranslated region 1322-base pair 3'-untranslated region including 20 nucleotides of poly A tail in the total length of 2873 base pairs. The deduced amino acid sequence showed 82% similarity to rat P-450ch7α. Unique amino acid residues were observed in putative binding domains for heme and steroid which are highly conserved in most steroidogenic P-450s. © 1990.
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页码：137 / 140
页数：4
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