MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF CDNA-ENCODING HUMAN CHOLESTEROL 7-ALPHA-HYDROXYLASE

被引:100
作者
NOSHIRO, M
OKUDA, K
机构
[1] Department of Biochemistry, School of Dentistry, Hiroshima University, Minami-ku, Hiroshima, 734
关键词
cDNA cloning; Cholesterol; 7α-hydroxylase; COS cell; Cytochrome P-450; Herne binding region; Steroid binding region;
D O I
10.1016/0014-5793(90)80992-R
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A complete cDNA clone encoding human cholesterol 7α-hydroxylase has been isolated using a rat P-450ch7α cDNA insert [(1989) FEBS Lett. 257, 97-100] as a probe and totally sequenced. The cDNA contained 1512-base pair open reading frame encoding 504 amino acid residues (Mr, 57630), 39-base pair 5'-untranslated region 1322-base pair 3'-untranslated region including 20 nucleotides of poly A tail in the total length of 2873 base pairs. The deduced amino acid sequence showed 82% similarity to rat P-450ch7α. Unique amino acid residues were observed in putative binding domains for heme and steroid which are highly conserved in most steroidogenic P-450s. © 1990.
引用
收藏
页码:137 / 140
页数:4
相关论文
共 20 条
[1]  
BINDER R, 1989, J BIOL CHEM, V264, P16910
[2]   NUCLEOTIDE-SEQUENCE ANALYSIS OF MURINE 21-HYDROXYLASE GENES - MUTATIONS AFFECTING GENE-EXPRESSION [J].
CHAPLIN, DD ;
GALBRAITH, LJ ;
SEIDMAN, JG ;
WHITE, PC ;
PARKER, KL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (24) :9601-9605
[3]   CYTOCHROME P450C17 (STEROID 17-ALPHA-HYDROXYLASE/17,20 LYASE) - CLONING OF HUMAN ADRENAL AND TESTIS CDNAS INDICATES THE SAME GENE IS EXPRESSED IN BOTH TISSUES [J].
CHUNG, BC ;
PICADOLEONARD, J ;
HANIU, M ;
BIENKOWSKI, M ;
HALL, PF ;
SHIVELY, JE ;
MILLER, WL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (02) :407-411
[4]   BILE-ACID METABOLISM [J].
DANIELSSON, H ;
SJOVALL, J .
ANNUAL REVIEW OF BIOCHEMISTRY, 1975, 44 :233-253
[5]  
GOTOH O, 1989, BASIS MECHANISMS REG, P196
[6]   UNIDIRECTIONAL DIGESTION WITH EXONUCLEASE-III CREATES TARGETED BREAKPOINTS FOR DNA SEQUENCING [J].
HENIKOFF, S .
GENE, 1984, 28 (03) :351-359
[7]   GENE STRUCTURE OF HUMAN CYTOCHROME-P-450(SCC), CHOLESTEROL DESMOLASE [J].
MOROHASHI, K ;
SOGAWA, K ;
OMURA, T ;
FUJIIKURIYAMA, Y .
JOURNAL OF BIOCHEMISTRY, 1987, 101 (04) :879-887
[8]   RAT TESTIS P-45017-ALPHA CDNA - THE DEDUCED AMINO-ACID SEQUENCE, EXPRESSION AND SECONDARY STRUCTURAL CONFIGURATION [J].
NAMIKI, M ;
KITAMURA, M ;
BUCZKO, E ;
DUFAU, ML .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 157 (02) :705-712
[9]   THE P450 SUPERFAMILY - UPDATED LISTING OF ALL GENES AND RECOMMENDED NOMENCLATURE FOR THE CHROMOSOMAL LOCI [J].
NEBERT, DW ;
NELSON, DR ;
ADESNIK, M ;
COON, MJ ;
ESTABROOK, RW ;
GONZALEZ, FJ ;
GUENGERICH, FP ;
GUNSALUS, IC ;
JOHNSON, EF ;
KEMPER, B ;
LEVIN, W ;
PHILLIPS, IR ;
SATO, R ;
WATERMAN, MR .
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1989, 8 (01) :1-13
[10]   MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF CDNA-ENCODING RAT ADRENAL CYTOCHROME-P-45011-BETA [J].
NONAKA, Y ;
MATSUKAWA, N ;
MOROHASHI, K ;
OMURA, T ;
OGIHARA, T ;
TERAOKA, H ;
OKAMOTO, M .
FEBS LETTERS, 1989, 255 (01) :21-26