CONDITIONAL HYPORECOMBINATION MUTANTS OF 3 REC GENES OF SACCHAROMYCES-CEREVISIAE

被引：12
作者：
ESPOSITO, MS
BROWN, JT
机构：
[1] Cell and Molecular Biology Division, Lawrence Berkeley Laboratory, 934-47A, University of California, Berkeley, 94720, CA
来源：
CURRENT GENETICS | 1990年 / 17卷 / 01期
关键词：
Gene conversion; REC genes; Recombination; Sporulation;
D O I：
10.1007/BF00313242
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
We have isolated and characterized three conditional hyporecombination mutants, rec1-1, rec3-1 and rec4-1, that define three REC genes of Saccharomyces cerevisiae required for spontaneous general mitotic interchromosomal recombination. Each MATa/MATα rec/rec diploid is deficient in mitotic single site gene conversion, intragenic recombination, intergenic recombination and sporulation at the restrictive temperature (36°C). The rec1-1 mutation also confers conditional enhanced sensitivity to the killing effects of X-rays. The rec1-1 and rec3-1 mutations have been mapped to chromosome VII. The rec1-1, rec3-1 and rec4-1 mutations exhibit complementation at 36°C for both mitotic recombination and sporulation. © 1990 Springer-Verlag.
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页码：7 / 12
页数：6
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